TY - JOUR
T1 - Microtubules and microfilaments in aging hamster embryo fibroblasts in vitro
AU - Raes, Martine
AU - Geuens, G.
AU - De Brabander, M.
AU - Remacle, J.
N1 - Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also vizualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
AB - Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also vizualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
UR - http://www.scopus.com/inward/record.url?scp=0020644045&partnerID=8YFLogxK
U2 - 10.1016/0531-5565(83)90035-9
DO - 10.1016/0531-5565(83)90035-9
M3 - Article
AN - SCOPUS:0020644045
SN - 0531-5565
VL - 18
SP - 241
EP - 254
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 3
ER -