Microtubules and microfilaments in aging hamster embryo fibroblasts in vitro

Martine Raes, G. Geuens, M. De Brabander, J. Remacle

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Microtubules and microfilaments were investigated in hamster lung fibroblasts, during their in vitro life-span. These cells show a senescence process characterized by a drastic phenotypic change, resulting in two phenotypes: the type 1 cells, characteristic of young cultures and the type 2 cells appearing progressively with culture passages. Microtubules and microfilaments were observed at the TEM and also vizualized by the unlabelled peroxidase-anti-peroxidase method. Moreover, the susceptibility of microtubules to nocodazole was tested in type 1 and 2 cells. We could not provide evidence for a different susceptibility to the drug. However the depolymerization wave occurred centripetally in type 1 cells whilst centrifugally in type 2 cells. These observations are discussed in relationship with the early arrest of division growth of the type 2 differentiated cells.
langue originaleAnglais
Pages (de - à)241-254
Nombre de pages14
journalExperimental Gerontology
Numéro de publication3
Les DOIs
Etat de la publicationPublié - 1 janv. 1983

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