TY - JOUR
T1 - Microinjection of antibodies against superoxide dismutase and glutathione peroxidase
AU - Michiels, Carine
AU - Raes, Martine
AU - Zachary, Marie-Denise
AU - Delaive, Edouard
AU - Remacle, José
PY - 1988
Y1 - 1988
N2 - Antibodies were prepared against glutathione peroxidase, superoxide dismutase, and catalase. Inhibition of the enzyme activity was obtained with anti-Gpx and anti-SOD antibodies but not with anti-CAT antibodies. The antibodies were then injected into human fibroblasts and bovine chondrocytes in culture either under normal conditions or under 1 atm of oxygen. The injected anti-Gpx and anti-SOD antibodies increased the mortality rate of the fibroblasts incubated under 1 atm of oxygen. However, when cells were incubated under normal atmosphere, anti-Gpx antibodies inhibited the division while anti-SOD antibodies increased this capacity. Anti-Gpx antibodies injected into chondrocytes decreased their viability. Injection of control antiserum had no effect. These data stress the primary importance of Gpx as antioxidant under all conditions and the relative efficiency of SOD according to the balance between the radical production and the activity of the other antioxidant systems.
AB - Antibodies were prepared against glutathione peroxidase, superoxide dismutase, and catalase. Inhibition of the enzyme activity was obtained with anti-Gpx and anti-SOD antibodies but not with anti-CAT antibodies. The antibodies were then injected into human fibroblasts and bovine chondrocytes in culture either under normal conditions or under 1 atm of oxygen. The injected anti-Gpx and anti-SOD antibodies increased the mortality rate of the fibroblasts incubated under 1 atm of oxygen. However, when cells were incubated under normal atmosphere, anti-Gpx antibodies inhibited the division while anti-SOD antibodies increased this capacity. Anti-Gpx antibodies injected into chondrocytes decreased their viability. Injection of control antiserum had no effect. These data stress the primary importance of Gpx as antioxidant under all conditions and the relative efficiency of SOD according to the balance between the radical production and the activity of the other antioxidant systems.
UR - http://www.scopus.com/inward/record.url?scp=0024215797&partnerID=8YFLogxK
U2 - 10.1016/0014-4827(88)90296-0
DO - 10.1016/0014-4827(88)90296-0
M3 - Article
C2 - 3191955
AN - SCOPUS:0024215797
VL - 179
SP - 581
EP - 589
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -