Résumé
The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.
langue originale | Anglais |
---|---|
Numéro d'article | 707515 |
journal | Frontiers in Microbiology |
Volume | 12 |
Les DOIs | |
Etat de la publication | Publié - 26 juil. 2021 |
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Dans: Frontiers in Microbiology, Vol 12, 707515, 26.07.2021.
Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs
TY - JOUR
T1 - Microbiome in Blood Samples From the General Population Recruited in the MARK-AGE Project
T2 - A Pilot Study
AU - D’Aquila, Patrizia
AU - Giacconi, Robertina
AU - Malavolta, Marco
AU - Piacenza, Francesco
AU - Bürkle, Alexander
AU - Villanueva, María Moreno
AU - Dollé, Martijn E.T.
AU - Jansen, Eugène
AU - Grune, Tilman
AU - Gonos, Efstathios S.
AU - Franceschi, Claudio
AU - Capri, Miriam
AU - Grubeck-Loebenstein, Beatrix
AU - Sikora, Ewa
AU - Toussaint, Olivier
AU - Debacq-Chainiaux, Florence
AU - Hervonen, Antti
AU - Hurme, Mikko
AU - Slagboom, P. Eline
AU - Schön, Christiane
AU - Bernhardt, Jürgen
AU - Breusing, Nicolle
AU - Passarino, Giuseppe
AU - Provinciali, Mauro
AU - Bellizzi, Dina
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Nature 486, 222–227. doi: 10.1038/nature11053 Conflict of Interest: CS and JB were employed by the company BioTeSys GmbH. The authors declare that this study received funding from the European Commission, the Italian Health Ministry and the nursing homes of SADEL S.p.A. The funder was not involved in the study design, collection, analysis, interpretation of data, the writing of this article or the decision to submit it for publication. Funding Information: We thank all study subjects for their willingness to participate. The work has been made possible by the collaboration with the nursing homes of SADEL S.p.A (San Teodoro, San Raffaele, Villa del Rosario, A.G.I srl, SAVELLI HOSPITAL., Casa di Cura Madonna dello Scoglio) in the frame of the agreement “SOLUZIONI INNOVATIVE PER L’INNALZAMENTO DELLA SALUTE E DELLA SICUREZZA DELLA POPOLAZIONE” with the University of Calabria. Funding. This work was supported by the European Commission (Project Full Name: European Study to Establish Biomarkers of Human Aging; Project Acronym: MARK-AGE; Project No: 200880) and in part by the Project: SInergie di RIcerca della Rete Aging (SIRI) by Italian Health Ministry. Funding Information: This work was supported by the European Commission (Project Full Name: European Study to Establish Biomarkers of Human Aging; Project Acronym: MARK-AGE; Project No: 200880) and in part by the Project: SInergie di RIcerca della Rete Aging (SIRI) by Italian Health Ministry. Publisher Copyright: © Copyright © 2021 D’Aquila, Giacconi, Malavolta, Piacenza, Bürkle, Villanueva, Dollé, Jansen, Grune, Gonos, Franceschi, Capri, Grubeck-Loebenstein, Sikora, Toussaint, Debacq-Chainiaux, Hervonen, Hurme, Slagboom, Schön, Bernhardt, Breusing, Passarino, Provinciali and Bellizzi.
PY - 2021/7/26
Y1 - 2021/7/26
N2 - The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.
AB - The presence of circulating microbiome in blood has been reported in both physiological and pathological conditions, although its origins, identities and function remain to be elucidated. This study aimed to investigate the presence of blood microbiome by quantitative real-time PCRs targeting the 16S rRNA gene. To our knowledge, this is the first study in which the circulating microbiome has been analyzed in such a large sample of individuals since the study was carried out on 1285 Randomly recruited Age-Stratified Individuals from the General population (RASIG). The samples came from several different European countries recruited within the EU Project MARK-AGE in which a series of clinical biochemical parameters were determined. The results obtained reveal an association between microbial DNA copy number and geographic origin. By contrast, no gender and age-related difference emerged, thus demonstrating the role of the environment in influencing the above levels independent of age and gender at least until the age of 75. In addition, a significant positive association was found with Free Fatty Acids (FFA) levels, leukocyte count, insulin, and glucose levels. Since these factors play an essential role in both health and disease conditions, their association with the extent of the blood microbiome leads us to consider the blood microbiome as a potential biomarker of human health.
KW - 16S rRNA gene
KW - aging
KW - blood microbiome
KW - free fatty acids
KW - geographic origin
KW - glucose
KW - insulin
KW - leukocytes
UR - http://www.scopus.com/inward/record.url?scp=85112222608&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2021.707515
DO - 10.3389/fmicb.2021.707515
M3 - Article
AN - SCOPUS:85112222608
SN - 1664-302X
VL - 12
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 707515
ER -