Meta-analysis and gene set analysis of archived microarrays suggest implication of the spliceosome in metastatic and hypoxic phenotypes

Bertrand De Meulder, Fabrice Berger, Eric Bareke, Sophie Depiereux, Carine Michiels, Eric Depiereux

Résultats de recherche: Contribution à un journal/une revueArticle

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Résumé

We propose to make use of the wealth of underused DNA chip data available in public repositories to study the molecular mechanisms behind the adaptation of cancer cells to hypoxic conditions leading to the metastatic phenotype. We have developed new bioinformatics tools and adapted others to identify with maximum sensitivity those genes which are expressed differentially across several experiments. The comparison of two analytical approaches, based on either Over Representation Analysis or Functional Class Scoring, by a meta-analysis-based approach, led to the retrieval of known information about the biological situation - thus validating the model - but also more importantly to the discovery of the previously unknown implication of the spliceosome, the cellular machinery responsible for mRNA splicing, in the development of metastasis.

langue originaleAnglais
Numéro d'articlee86699
Nombre de pages13
journalPLoS ONE
Volume9
Numéro de publication1
Les DOIs
étatPublié - 2014

Empreinte digitale

spliceosomes
information retrieval
Spliceosomes
Information Storage and Retrieval
Microarray Analysis
Bioinformatics
Microarrays
Oligonucleotide Array Sequence Analysis
Computational Biology
meta-analysis
bioinformatics
anaerobic conditions
metastasis
Machinery
Meta-Analysis
Genes
Cells
Neoplasm Metastasis
Phenotype
phenotype

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Meta-analysis and gene set analysis of archived microarrays suggest implication of the spliceosome in metastatic and hypoxic phenotypes. / De Meulder, Bertrand; Berger, Fabrice; Bareke, Eric; Depiereux, Sophie; Michiels, Carine; Depiereux, Eric.

Dans: PLoS ONE, Vol 9, Numéro 1, e86699, 2014.

Résultats de recherche: Contribution à un journal/une revueArticle

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AU - Michiels, Carine

AU - Depiereux, Eric

PY - 2014

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