TY - JOUR
T1 - Marek's disease virus microRNA designated Mdv1-pre-miR-M4 targets both cellular and viral genes
AU - Muylkens, Benoît
AU - Coupeau, Damien
AU - Dambrine, Ginette
AU - Trapp, Sascha
AU - Rasschaert, Denis
PY - 2010/11
Y1 - 2010/11
N2 - Mdv1-miR-M4 is one of 25 microRNAs (miRNAs) expressed by Marek's disease virus (MDV-1), an oncogenic alphaherpesvirus that induces fatal T-cell lymphoma in chickens. Mdv1-miR-M4 was shown to be the second functional viral ortholog of miR-155, a cellular miRNA that plays a crucial role in several physiological and pathological processes in lymphocyte biology. In this study, we investigated a panel of putative mdv1-miR-M4 targets involved in gene networks affecting both cellular and viral life cycles. Using luciferase reporter assays, we showed that mdv1-miR-M4-5P and miR-155 efficiently targeted a common set of 3′ untranslated regions (3′UTR) of six cellular genes (GPM6B, RREB1, c-Myb, MAP3K7IP2, PU. 1 and C/EBP). In addition, we also investigated the interactions between mdv1-miR-M4-5P and mdv1-miR-M43P and viral mRNAs encoding UL28 and UL32 in both reporter and western blot assays. Mdv1-miR-M4 specifically inhibited the translation of these two viral proteins, which are involved in the cleavage/packaging of herpesvirus DNA. © 2010 Springer-Verlag.
AB - Mdv1-miR-M4 is one of 25 microRNAs (miRNAs) expressed by Marek's disease virus (MDV-1), an oncogenic alphaherpesvirus that induces fatal T-cell lymphoma in chickens. Mdv1-miR-M4 was shown to be the second functional viral ortholog of miR-155, a cellular miRNA that plays a crucial role in several physiological and pathological processes in lymphocyte biology. In this study, we investigated a panel of putative mdv1-miR-M4 targets involved in gene networks affecting both cellular and viral life cycles. Using luciferase reporter assays, we showed that mdv1-miR-M4-5P and miR-155 efficiently targeted a common set of 3′ untranslated regions (3′UTR) of six cellular genes (GPM6B, RREB1, c-Myb, MAP3K7IP2, PU. 1 and C/EBP). In addition, we also investigated the interactions between mdv1-miR-M4-5P and mdv1-miR-M43P and viral mRNAs encoding UL28 and UL32 in both reporter and western blot assays. Mdv1-miR-M4 specifically inhibited the translation of these two viral proteins, which are involved in the cleavage/packaging of herpesvirus DNA. © 2010 Springer-Verlag.
UR - http://www.scopus.com/inward/record.url?scp=78149409469&partnerID=8YFLogxK
U2 - 10.1007/s00705-010-0777-y
DO - 10.1007/s00705-010-0777-y
M3 - Article
C2 - 20680360
VL - 155
SP - 1823
EP - 1837
JO - Archives of virology
JF - Archives of virology
IS - 11
ER -