TY - JOUR
T1 - Mapping sugar beet pectin acetylation pattern
AU - Ralet, M.-C.
AU - Cabrera Pino, Juan Carlos
AU - Bonnin, E.
AU - Quéméner, B.
AU - Hellìn, P.
AU - Thibault, J.-F.
PY - 2005/8/1
Y1 - 2005/8/1
N2 - Homogalacturonan-derived partly methylated and/or acetylated oligogalacturonates were recovered after enzymatic hydrolysis (endo-polygalacturonase + pectin methyl esterase + side-chain degrading enzymes) of sugar beet pectin followed by anion-exchange and size exclusion chromatography. Around 90% of the GalA and 75% of the acetyl groups present in the initial sugar beet pectin were recovered as homogalacturonan-derived oligogalacturonates, the remaining GalA and acetyl belonging to rhamnogalacturonic regions. Around 50% of the acetyl groups present in sugar beet homogalacturonans were recovered as partly methylated and/or acetylated oligogalacturonates of degree of polymerisation ≤5 whose structures were determined by electrospray ionization ion trap mass spectrometry (ESI-IT-MS ). 2-O-acetyl- and 3-O-acetyl-GalA were detected in roughly similar amounts but 2,3-di-O-acetylation was absent. Methyl-esterified GalA residues occurred mainly upstream 2-O-acetyl GalA. Oligogalacturonates containing GalA residues that are at once methyl- and acetyl-esterified were recovered in very limited amounts. A tentative mapping of the distribution of acetyl and methyl esters within sugar beet homogalacturonans is proposed. Unsubstituted GalA residues are likely to be present in limited amounts (∼10% of total GalA residues), due to the fact that methyl and acetyl groups are assumed to be most often not carried by the same residues.
AB - Homogalacturonan-derived partly methylated and/or acetylated oligogalacturonates were recovered after enzymatic hydrolysis (endo-polygalacturonase + pectin methyl esterase + side-chain degrading enzymes) of sugar beet pectin followed by anion-exchange and size exclusion chromatography. Around 90% of the GalA and 75% of the acetyl groups present in the initial sugar beet pectin were recovered as homogalacturonan-derived oligogalacturonates, the remaining GalA and acetyl belonging to rhamnogalacturonic regions. Around 50% of the acetyl groups present in sugar beet homogalacturonans were recovered as partly methylated and/or acetylated oligogalacturonates of degree of polymerisation ≤5 whose structures were determined by electrospray ionization ion trap mass spectrometry (ESI-IT-MS ). 2-O-acetyl- and 3-O-acetyl-GalA were detected in roughly similar amounts but 2,3-di-O-acetylation was absent. Methyl-esterified GalA residues occurred mainly upstream 2-O-acetyl GalA. Oligogalacturonates containing GalA residues that are at once methyl- and acetyl-esterified were recovered in very limited amounts. A tentative mapping of the distribution of acetyl and methyl esters within sugar beet homogalacturonans is proposed. Unsubstituted GalA residues are likely to be present in limited amounts (∼10% of total GalA residues), due to the fact that methyl and acetyl groups are assumed to be most often not carried by the same residues.
UR - http://www.scopus.com/inward/record.url?scp=23144431608&partnerID=8YFLogxK
U2 - 10.1016/j.phytochem.2005.06.003
DO - 10.1016/j.phytochem.2005.06.003
M3 - Article
AN - SCOPUS:23144431608
SN - 0031-9422
VL - 66
SP - 1832
EP - 1843
JO - Phytochemistry
JF - Phytochemistry
IS - 15
ER -