@article{70f92623f7584cf4a484df2a7bb520bb,
title = "Localized incorporation of outer membrane components in the pathogen Brucella abortus",
abstract = "The zoonotic pathogen Brucella abortus is part of the Rhizobiales, which are alpha-proteobacteria displaying unipolar growth. Here, we show that this bacterium exhibits heterogeneity in its outer membrane composition, with clusters of rough lipopolysaccharide co-localizing with the essential outer membrane porin Omp2b, which is proposed to allow facilitated diffusion of solutes through the porin. We also show that the major outer membrane protein Omp25 and peptidoglycan are incorporated at the new pole and the division site, the expected growth sites. Interestingly, lipopolysaccharide is also inserted at the same growth sites. The absence of long-range diffusion of main components of the outer membrane could explain the apparent immobility of the Omp2b clusters, as well as unipolar and mid-cell localizations of newly incorporated outer membrane proteins and lipopolysaccharide. Unipolar growth and limited mobility of surface structures also suggest that new surface variants could arise in a few generations without the need of diluting pre-existing surface antigens.",
keywords = "Brucella, lipopolysaccharide, outer membrane, unipolar growth",
author = "Victoria Vassen and Claire Valotteau and C{\'e}cile Feuillie and C{\'e}cile Formosa-Dague and Dufr{\^e}ne, {Yves F} and {De Bolle}, Xavier",
note = "Funding Information: We thank Ignacio Moriy{\'o}n, Raquel Conde {\'A}lvarez, Francesco Renzi, Jean-Yves Matroule, and Jean-Fran{\c c}ois Collet for stimulating and helpful discussion. We thank St{\'e}phane Vincent and his team for the synthesis of HADA and Boris Vauzeilles for providing Kdo-N3. We thank K{\'e}vin Willemart for the technical assistance in flow cytometry and Beatriz Izquierdo Lafuente for providing the B. abortus Δomp25 strain. This research has been funded by grants from Fonds de la Recherche Scientifique-Fonds National de la Recherche Scientifique (FRS-FNRS, http://www.fnrs.be) (PDR T.0053.13 and PDR Brucell-cycle T.0060.15, and CDR J.0091.14) to X. De Bolle. The work was also funded by a grant from Concerted Research Actions (17/22-087) of the F{\'e}d{\'e}ration Wallonie-Bruxelles, to J.-F. Collet, P. Soumillion and X. De Bolle. We thank UNamur (https://www.un amur.be/) for financial and logistic support. V. Vassen is supported by a Ph.D. grant from FRIA (FRS-FNRS). Work at the Universit{\'e} Catholique de Louvain was supported by the European Research Council (ERC) under the European Union{\textquoteright}s Horizon 2020 research and innovation program (grant agreement 693630), the FRS-FNRS, the FNRS-WELBIO (grant WELBIO-CR-2015A-05), and the Research Department of the Communaut{\'e} Fran{\c c}aise de Belgique (Concerted Research Action). Y.F. Dufr{\^e}ne is a Research Director at FNRS. Funding Information: We thank Ignacio Moriy?n, Raquel Conde ?lvarez, Francesco Renzi, Jean-Yves Matroule, and Jean-Fran?ois Collet for stimulating and helpful discussion. We thank St?phane Vincent and his team for the synthesis of HADA and Boris Vauzeilles for providing Kdo-N3. We thank K?vin Willemart for the technical assistance in flow cytometry and Beatriz Izquierdo Lafuente for providing the B.?abortus ?omp25 strain. This research has been funded by grants from Fonds de la Recherche Scientifique-Fonds National de la Recherche Scientifique (FRS-FNRS, http://www.fnrs.be) (PDR T.0053.13 and PDR Brucell-cycle T.0060.15, and CDR J.0091.14) to X. De Bolle. The work was also funded by a grant from Concerted Research Actions (17/22-087) of the F?d?ration Wallonie-Bruxelles, to J.-F. Collet, P. Soumillion and X. De Bolle. We thank UNamur (https://www.unamur.be/) for financial and logistic support. V. Vassen is supported by a Ph.D. grant from FRIA (FRS-FNRS). Work at the Universit? Catholique de Louvain was supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement 693630), the FRS-FNRS, the FNRS-WELBIO (grant WELBIO-CR-2015A-05), and the Research Department of the Communaut? Fran?aise de Belgique (Concerted Research Action). Y.F. Dufr?ne is a Research Director at FNRS. Publisher Copyright: {\textcopyright} 2019 The Authors Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
month = mar,
day = "1",
doi = "10.15252/embj.2018100323",
language = "English",
volume = "38",
journal = "The EMBO journal",
issn = "0261-4189",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "5",
}