Lipopolysaccharide biosynthesis and traffic in the envelope of the pathogen Brucella abortus

Caroline Servais, Victoria Vassen, Audrey Verhaeghe, Nina Küster, Elodie Carlier, Léa Phégnon, Aurélie Mayard, Nicolas Auberger, Stéphane Vincent, Xavier De Bolle

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

39 Téléchargements (Pure)

Résumé

Lipopolysaccharide is essential for most Gram-negative bacteria as it is a main component of the outer membrane. In the pathogen Brucella abortus, smooth lipopolysaccharide containing the O-antigen is required for virulence. Being part of the Rhizobiales, Brucella spp. display unipolar growth and lipopolysaccharide was shown to be incorporated at the active growth sites, i.e. the new pole and the division site. By localizing proteins involved in the lipopolysaccharide transport across the cell envelope, from the inner to the outer membrane, we show that the lipopolysaccharide incorporation sites are determined by the inner membrane complex of the lipopolysaccharide transport system. Moreover, we identify the main O-antigen ligase of Brucella spp. involved in smooth lipopolysaccharide synthesis. Altogether, our data highlight a layer of spatiotemporal organization of the lipopolysaccharide biosynthesis pathway and identify an original class of bifunctional O-antigen ligases.

langue originaleAnglais
Numéro d'article911
Pages (de - à)911
journalNature Communications
Volume14
Numéro de publication1
Les DOIs
Etat de la publicationPublié - 17 févr. 2023

Empreinte digitale

Examiner les sujets de recherche de « Lipopolysaccharide biosynthesis and traffic in the envelope of the pathogen Brucella abortus ». Ensemble, ils forment une empreinte digitale unique.

Contient cette citation