LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation

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The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.

langue originaleAnglais
Numéro d'article455101
journalNanotechnology
Volume27
Numéro de publication45
Les DOIs
étatPublié - 2016

Empreinte digitale

Proton irradiation
Gold
Energy transfer
Nanoparticles
Protons
Oncology
Proton beams
Amplification
Reactive Oxygen Species
Physical properties
Cells

Citer ceci

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title = "LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation",
abstract = "The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.",
keywords = "A431 cells, cell uptake, gold nanoparticles, proton irradiation, radiosensitization",
author = "Sha Li and S{\'e}bastien Penninckx and Linda Karmani and Heuskin, {Anne Catherine} and Kassandra Watillon and Riccardo Marega and Jerome Zola and Valentina Corvaglia and Geraldine Genard and Bernard Gallez and Olivier Feron and Philippe Martinive and Davide Bonifazi and Carine Michiels and St{\'e}phane Lucas",
year = "2016",
doi = "10.1088/0957-4484/27/45/455101",
language = "English",
volume = "27",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "45",

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T1 - LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation

AU - Li, Sha

AU - Penninckx, Sébastien

AU - Karmani, Linda

AU - Heuskin, Anne Catherine

AU - Watillon, Kassandra

AU - Marega, Riccardo

AU - Zola, Jerome

AU - Corvaglia, Valentina

AU - Genard, Geraldine

AU - Gallez, Bernard

AU - Feron, Olivier

AU - Martinive, Philippe

AU - Bonifazi, Davide

AU - Michiels, Carine

AU - Lucas, Stéphane

PY - 2016

Y1 - 2016

N2 - The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.

AB - The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.

KW - A431 cells

KW - cell uptake

KW - gold nanoparticles

KW - proton irradiation

KW - radiosensitization

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