Résumé
The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
| langue originale | Anglais |
|---|---|
| Numéro d'article | 455101 |
| journal | Nanotechnology |
| Volume | 27 |
| Numéro de publication | 45 |
| Les DOIs | |
| état | Publié - 2016 |
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LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation. / Li, Sha; Penninckx, Sébastien; Karmani, Linda; Heuskin, Anne Catherine; Watillon, Kassandra; Marega, Riccardo; Zola, Jerome; Corvaglia, Valentina; Genard, Geraldine; Gallez, Bernard; Feron, Olivier; Martinive, Philippe; Bonifazi, Davide; Michiels, Carine; Lucas, Stéphane.
Dans: Nanotechnology, Vol 27, Numéro 45, 455101, 2016.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation
AU - Li, Sha
AU - Penninckx, Sébastien
AU - Karmani, Linda
AU - Heuskin, Anne Catherine
AU - Watillon, Kassandra
AU - Marega, Riccardo
AU - Zola, Jerome
AU - Corvaglia, Valentina
AU - Genard, Geraldine
AU - Gallez, Bernard
AU - Feron, Olivier
AU - Martinive, Philippe
AU - Bonifazi, Davide
AU - Michiels, Carine
AU - Lucas, Stéphane
PY - 2016
Y1 - 2016
N2 - The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
AB - The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
KW - A431 cells
KW - cell uptake
KW - gold nanoparticles
KW - proton irradiation
KW - radiosensitization
UR - http://www.scopus.com/inward/record.url?scp=84991710999&partnerID=8YFLogxK
U2 - 10.1088/0957-4484/27/45/455101
DO - 10.1088/0957-4484/27/45/455101
M3 - Article
VL - 27
JO - Nanotechnology
JF - Nanotechnology
SN - 0957-4484
IS - 45
M1 - 455101
ER -