Projets par an
Résumé
The development of new modalities and protocols is of major interest to improve the outcome of cancer treatment. Given the appealing physical properties of protons and the emerging evidence of biological relevance of the use of gold nanoparticles (GNPs), the radiosensitization effects of GNPs (5 or 10 nm) have been investigated in vitro in combination with a proton beam of different linear energy transfer (LET). After the incubation with GNPs for 24 h, nanoparticles were observed in the cytoplasm of A431 cells exposed to 10 nm GNPs, and in the cytoplasm as well as the nucleus of cells exposed to 5 nm GNPs. Cell uptake of 0.05 mg ml-1 of GNPs led to 0.78 pg Au/cell and 0.30 pg Au/cell after 24 h incubation for 10 and 5 nm GNPs respectively. A marked radiosensitization effect of GNPs was observed with 25 keV μm-1 protons, but not with 10 keV μm-1 protons. This effect was more pronounced for 10 nm GNPs than for 5 nm GNPs. By using a radical scavenger, a major role of reactive oxygen species in the amplification of the death of irradiated cell was identified. All together, these results open up novel perspectives for using high-Z metallic NPs in protontherapy.
langue originale | Anglais |
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Numéro d'article | 455101 |
journal | Nanotechnology |
Volume | 27 |
Numéro de publication | 45 |
Les DOIs | |
Etat de la publication | Publié - 2016 |
Empreinte digitale Examiner les sujets de recherche de « LET-dependent radiosensitization effects of gold nanoparticles for proton irradiation ». Ensemble, ils forment une empreinte digitale unique.
Projets
- 1 Terminé
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THERAPLUS: Amélioration de la radiothérapie externe grâce à l'utilisation de nano-objets métalliques
Heuskin, A., Lucas, S., MICHIELS, C., Feron, O., Gallez, B. & MARTINIVE, P.
1/05/14 → 1/05/17
Projet: Recherche
Équipement
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Microscope confocal
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Equipement
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Microscope électronique à transmission
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Equipement
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Morphologie - Imagerie
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Plateforme technolgique
Activités
- 1 Participation à une conférence, un congrès
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63rd annual Congress of the Radiation Research Society
Stéphane Lucas (Poster)
15 oct. 2017 → 18 oct. 2017Activité: Types de Participation ou d'organisation d'un événement › Participation à une conférence, un congrès