Knocking down p53 with siRNA does not affect the overexpression of p21 after exposure of IMR-90 hTERT fibroblasts to a sublethal concentration of HO leading to premature senescence

Résultats de recherche: Contribution dans un livre/un catalogue/un rapport/dans les actes d'une conférenceChapitre (revu par des pairs)

Résumé

Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.
langue originaleAnglais
titreAnnals of the New York Academy of Sciences
Pages316-322
Nombre de pages7
Volume1100
Les DOIs
étatPublié - 1 avr. 2007

Empreinte digitale

Cell Aging
Fibroblasts
Galactosidases
RNA
Activity Cycles
Cell Shape
Telomerase
Cell Cycle Checkpoints
Diploidy
Biomarkers
Messenger RNA
DNA

Citer ceci

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title = "Knocking down p53 with siRNA does not affect the overexpression of p21 after exposure of IMR-90 hTERT fibroblasts to a sublethal concentration of HO leading to premature senescence",
abstract = "Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.",
author = "S. Zdanov and F. Debacq-Chainiaux and O. Toussaint",
year = "2007",
month = "4",
day = "1",
doi = "10.1196/annals.1395.034",
language = "English",
isbn = "9781573316798",
volume = "1100",
pages = "316--322",
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Knocking down p53 with siRNA does not affect the overexpression of p21 after exposure of IMR-90 hTERT fibroblasts to a sublethal concentration of HO leading to premature senescence. / Zdanov, S.; Debacq-Chainiaux, F.; Toussaint, O.

Annals of the New York Academy of Sciences. Vol 1100 2007. p. 316-322.

Résultats de recherche: Contribution dans un livre/un catalogue/un rapport/dans les actes d'une conférenceChapitre (revu par des pairs)

TY - CHAP

T1 - Knocking down p53 with siRNA does not affect the overexpression of p21 after exposure of IMR-90 hTERT fibroblasts to a sublethal concentration of HO leading to premature senescence

AU - Zdanov, S.

AU - Debacq-Chainiaux, F.

AU - Toussaint, O.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.

AB - Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.

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BT - Annals of the New York Academy of Sciences

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