TY - CHAP
T1 - Knocking down p53 with siRNA does not affect the overexpression of p21 after exposure of IMR-90 hTERT fibroblasts to a sublethal concentration of HO leading to premature senescence
AU - Zdanov, S.
AU - Debacq-Chainiaux, Florence
AU - Toussaint, Olivier
PY - 2007/4/1
Y1 - 2007/4/1
N2 - Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.
AB - Premature senescence of IMR-90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H O, with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence-associated β-galactosidase (SA β-gal) activity, and cell cycle arrest. The induction of stress-induced premature senescence (SIPS) was associated with a transient increase in DNA-binding activity of p53 and an increased expression of p21. p53 small interferent RNA (siRNA) affected the basal level of p21 mRNA but did not affect the overexpression of p21 after stress. This siRNA approach confirms previous results obtained with other methods.
UR - http://www.scopus.com/inward/record.url?scp=34249656140&partnerID=8YFLogxK
U2 - 10.1196/annals.1395.034
DO - 10.1196/annals.1395.034
M3 - Chapter (peer-reviewed)
AN - SCOPUS:34249656140
SN - 9781573316798
VL - 1100
SP - 316
EP - 322
BT - Annals of the New York Academy of Sciences
ER -