Keratinocytes activated by IL-4/IL-13 express IL-2Rγ with consequences on epidermal barrier function

Audrey Progneaux, Celine Evrard, Valerie De Glas, Alix Fontaine, Evelyne DE VUYST, Arjen F. Nikkels, Vicente Garcia-Gonzalez, Laure Dumoutier, Catherine Lambert De Rouvroit, Yves Poumay

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Atopic dermatitis (AD) is a Th2-type inflammatory disease characterized by an alteration of epidermal barrier following the release of IL-4 and IL-13. These cytokines activate type II IL-4Rα/IL-13Rα1 receptors in the keratinocyte. Whilst IL-2Rγ, that forms type I receptor for IL-4, is only expressed in haematopoietic cells, recent studies suggest its induction in keratinocytes, which questions about its role. We studied expression of IL-2Rγ in keratinocytes and its role in alteration of keratinocyte function and epidermal barrier. IL-2Rγ expression in keratinocytes was studied using both reconstructed human epidermis (RHE) exposed to IL-4/IL-13 and AD skin. IL-2Rγ induction by type II receptor has been analyzed using JAK inhibitors and RHE knockout (KO) for IL13RA1. IL-2Rγ function was investigated in RHE KO for IL2RG. In RHE, IL-4/IL-13 induce expression of IL-2Rγ at the mRNA and protein levels. Its mRNA expression is also visualized in keratinocytes of lesional AD skin. IL-2Rγ expression is low in RHE treated with JAK inhibitors and absent in RHE KO for IL13RA1. Exposure to IL-4/IL-13 alters epidermal barrier, but this alteration is absent in RHE KO for IL2RG. A more important induction of IL-13Rα2 is reported in RHE KO for IL2RG than in not edited RHE. These results demonstrate IL-2Rγ induction in keratinocytes through activation of type II receptor. IL-2Rγ is involved in the alteration of the epidermal barrier and in the regulation of IL-13Rα2 expression. Observation of IL-2Rγ expression by keratinocytes inside AD lesional skin suggests a role for this receptor subunit in the disease.

langue originaleAnglais
Pages (de - à)660-670
Nombre de pages11
journalExperimental dermatology
Volume32
Numéro de publication5
Date de mise en ligne précoce19 janv. 2023
Les DOIs
Etat de la publicationPublié - mai 2023

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