Résumé
Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10<sup>- 8</sup> M-1.0 × 10<sup>- 6</sup> M and 2.0 × 10<sup>- 6</sup> M-1.0 × 10<sup>- 5</sup> M with detection limit (4.80 ± 0.35) × 10<sup>- 8</sup> M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.
| langue | Anglais |
|---|---|
| Pages | 328-337 |
| Nombre de pages | 10 |
| journal | Materials science and engineering C |
| Volume | 57 |
| Les DOIs | |
| état | Publié - 17 août 2015 |
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Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and l-tyrosine. / Bhakta, Arvind Kumar; Mascarenhas, Ronald J.; D'Souza, Ozma J.; Satpati, Ashis K.; Detriche, Simon; Mekhalif, Zineb; Delhalle, Joseph.
Dans: Materials science and engineering C, Vol 57, 17.08.2015, p. 328-337.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode as an electrochemical sensor for the simultaneous determination of uric acid in the presence of ascorbic acid, dopamine and l-tyrosine
AU - Bhakta, Arvind Kumar
AU - Mascarenhas, Ronald J.
AU - D'Souza, Ozma J.
AU - Satpati, Ashis K.
AU - Detriche, Simon
AU - Mekhalif, Zineb
AU - Delhalle, Joseph
PY - 2015/8/17
Y1 - 2015/8/17
N2 - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10- 8 M-1.0 × 10- 6 M and 2.0 × 10- 6 M-1.0 × 10- 5 M with detection limit (4.80 ± 0.35) × 10- 8 M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.
AB - Iron nanoparticles decorated multi-wall carbon nanotubes modified carbon paste electrode (Fe-MWCNTs/MCPE) was prepared by bulk-modification method. The electrochemical impedance spectroscopy (EIS) suggests least charge transfer resistance at the modified electrode. The electrochemical behavior of UA was studied in 0.1 M phosphate buffer solution (PBS) of pH 3.0 using cyclic voltammetry (CV) while differential pulse voltammetry (DPV) was used for quantification. The spectroelectrochemial study of oxidation of UA at Fe-MWCNTs/MCPE showed a decrease in the absorbance of two peaks with time, which are ascribed to π to π∗ and n to π∗ transitions. Under optimum condition, the DPV response offered two linear dynamic ranges for UA in the concentration range 7.0 × 10- 8 M-1.0 × 10- 6 M and 2.0 × 10- 6 M-1.0 × 10- 5 M with detection limit (4.80 ± 0.35) × 10- 8 M (S/N = 3). The practical analytical application of this sensor was successfully evaluated by determination of spiked UA in clinical samples, such as human blood serum and urine with good percentage recovery. The proposed electrochemical sensor offers a simple, reliable, rapid, reproducible and cost effective analysis of a quaternary mixture of biomolecules containing AA, DA, UA and Tyr which was free from mutual interferences.
KW - Iron nanoparticles decorated multi-wall carbon nanotubes
KW - Uric acid
KW - Ascorbic acid
KW - Dopamine
KW - L-Tyrosine
KW - Differential pulse voltammetry
UR - http://www.scopus.com/inward/record.url?scp=84939149404&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2015.08.003
DO - 10.1016/j.msec.2015.08.003
M3 - Article
VL - 57
SP - 328
EP - 337
JO - Materials science and engineering C
T2 - Materials science and engineering C
JF - Materials science and engineering C
SN - 0928-4931
ER -