Iron Ladies: How Desiccated Asexual Rotifer Adineta vaga Deal With X-Rays and Heavy Ions?

Boris Hespeels, Sébastien Penninckx, Valérie Cornet, Lucie Bruneau, Cécile Bopp, Véronique Baumlé, Baptiste Redivo, Anne-Catherine Heuskin, Ralf Moeller, Akira Fujimori, Stéphane Lucas, Karine Van Doninck

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Space exposure experiments from the last 15 years have unexpectedly shown that several terrestrial organisms, including some multi-cellular species, are able to survive in open space without protection. The robustness of bdelloid rotifers suggests that these tiny creatures can possibly be added to the still restricted list of animals that can deal with the exposure to harsh condition of space. Bdelloids are one of the smallest animals on Earth. Living all over the world, mostly in semi-terrestrial environments, they appear to be extremely stress tolerant. Their desiccation tolerance at any stage of their life cycle is known to confer tolerance to a variety of stresses including high doses of radiation and freezing. In addition, they constitute a major scandal in evolutionary biology due to the putative absence of sexual reproduction for at least 60 million years. Adineta vaga, with its unique characteristics and a draft genome available, was selected by ESA (European Space Agency) as a model system to study extreme resistance of organisms exposed to space environment. In this manuscript, we documented the resistance of desiccated A. vaga individuals exposed to increasing doses of X-ray, protons and Fe ions. Consequences of exposure to different sources of radiation were investigated in regard to the cellular type including somatic (survival assay) and germinal cells (fertility assay). Then, the capacity of A. vaga individuals to repair DNA DSB induced by different source of radiation was investigated. Bdelloid rotifers represent a promising model in order to investigate damage induced by high or low LET radiation. The possibility of exposure both on hydrated or desiccated specimens may help to decipher contribution of direct and indirect radiation damage on biological processes. Results achieved through this study consolidate our knowledge about the radioresistance of A. vaga and improve our capacity to compare extreme resistance against radiation among living organisms including metazoan.

langue originaleAnglais
Numéro d'article1792
Pages (de - à)1792
journalFrontiers in Microbiology
Volume11
Date de mise en ligne précoce31 juil. 2020
Les DOIs
Etat de la publicationPublié - 2020

Financement

R. Coos, R. Tonneau, and T. Tabarrant are strongly acknowledged for their assistance at any time during X-rays and proton exposure sessions. The authors also thank the European Space Agency (ESA) and the Belgian Federal Science Policy Office (BELSPO) for their support in the framework of the PRODEX Program. Funding. The authors were supported by the European Space Agency (ESA) and the Belgian Federal Science Policy Office (BELSPO) in the framework of the PRODEX Programme. Fe radiation was part of STARLIFE astrobiology inter-comparison experiments at HIMAC (led by RM). STARLIFE was supported by the MEXT Grant-in-Aid for Scientific Research on Innovative Areas ?Living in Space? (Grant Numbers: 15H05935 and 15K21745). RM was supported by the DLR grant FuE-Projekt ?ISS LIFE? (Programm RF-FuW, TP 475). SP was funded by the Walloon Region (PROTHERWAL, grant n? 7289).

Bailleurs de fondsNuméro du bailleur de fonds
PROTHERWAL
Walloon Regionn◦ 7289
Horizon 2020 Framework Programme725998
Division of Loan RepaymentTP 475
European Space Agency
Ministry of Education, Culture, Sports, Science and Technology15K21745, 15H05935
Belgian Science Policy Office
Deutsches Zentrum fur Luft- Und Raumfahrt

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