Intrinsic antibacterial activity of nanoparticles made of β-cyclodextrins potentiates their effect as drug nanocarriers against tuberculosis

Arnaud Machelart, Giuseppina Salzano, Xue Li, Aurore Demars, Anne Sophie Debrie, Mario Menendez-Miranda, Elisabetta Pancani, Samuel Jouny, Eik Hoffmann, Nathalie Deboosere, Imène Belhaouane, Carine Rouanet, Sophie Simar, Smaïl Talahari, Valerie Giannini, Baptiste Villemagne, Marion Flipo, Roland Brosch, Fabrice Nesslany, Benoit DeprezEric Muraille, Camille Locht, Alain R. Baulard, Nicolas Willand, Laleh Majlessi, Ruxandra Gref, Priscille Brodin

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

44 Téléchargements (Pure)

Résumé

Multi-drug-resistant tuberculosis (TB) is a major public health problem, concerning about half a million cases each year. Patients hardly adhere to the current strict treatment consisting of more than 10,000 tablets over a 2-year period. There is a clear need for efficient and better formulated medications. We have previously shown that nanoparticles made of cross-linked poly-β-cyclodextrins (pβCD) are efficient vehicles for pulmonary delivery of powerful combinations of anti-TB drugs. Here, we report that in addition to being efficient drug carriers, pβCD nanoparticles are endowed with intrinsic antibacterial properties. Empty pβCD nanoparticles are able to impair Mycobacterium tuberculosis (Mtb) establishment after pulmonary administration in mice. pβCD hamper colonization of macrophages by Mtb by interfering with lipid rafts, without inducing toxicity. Moreover, pβCD provoke macrophage apoptosis, leading to depletion of infected cells, thus creating a lung microenvironment detrimental to Mtb persistence. Taken together, our results suggest that pβCD nanoparticles loaded or not with antibiotics have an antibacterial action on their own and could be used as a carrier in drug regimen formulations effective against TB.

langue originaleAnglais
Pages (de - à)3992-4007
Nombre de pages16
journalACS nano
Volume13
Numéro de publication4
Les DOIs
Etat de la publicationPublié - 23 avr. 2019

Empreinte digitale

Examiner les sujets de recherche de « Intrinsic antibacterial activity of nanoparticles made of β-cyclodextrins potentiates their effect as drug nanocarriers against tuberculosis ». Ensemble, ils forment une empreinte digitale unique.

Contient cette citation