Résumé
Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.
| langue originale | Anglais |
|---|---|
| Pages (de - à) | 1613-1622 |
| Nombre de pages | 10 |
| journal | Applied Surface Science |
| Volume | 396 |
| Les DOIs | |
| état | Publié - 28 févr. 2017 |
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Interaction of phosphorylcholine with fibronectin coatings : Surface characterization and biological performances. / Montaño-Machado, Vanessa; Noël, Céline; Chevallier, Pascale; Turgeon, Stéphane; Houssiau, Laurent; Pauthe, Emmanuel; Pireaux, Jean-Jacques; Mantovani, Diego.
Dans: Applied Surface Science, Vol 396, 28.02.2017, p. 1613-1622.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Interaction of phosphorylcholine with fibronectin coatings
T2 - Surface characterization and biological performances
AU - Montaño-Machado, Vanessa
AU - Noël, Céline
AU - Chevallier, Pascale
AU - Turgeon, Stéphane
AU - Houssiau, Laurent
AU - Pauthe, Emmanuel
AU - Pireaux, Jean-Jacques
AU - Mantovani, Diego
PY - 2017/2/28
Y1 - 2017/2/28
N2 - Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.
AB - Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.
KW - Biomaterials
KW - Endothelialization
KW - Fibronectin
KW - Grafting
KW - Hemocompatibility
KW - Phosphorylcholine
UR - http://www.scopus.com/inward/record.url?scp=85006858085&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2016.11.224
DO - 10.1016/j.apsusc.2016.11.224
M3 - Article
VL - 396
SP - 1613
EP - 1622
JO - Appl. Surface Science
JF - Appl. Surface Science
SN - 0169-4332
ER -