Interaction of phosphorylcholine with fibronectin coatings: Surface characterization and biological performances

Vanessa Montaño-Machado, Céline Noël, Pascale Chevallier, Stéphane Turgeon, Laurent Houssiau, Emmanuel Pauthe, Jean-Jacques Pireaux, Diego Mantovani

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.

langueAnglais
Pages1613-1622
Nombre de pages10
journalApplied Surface Science
Volume396
Les DOIs
étatPublié - 28 févr. 2017

Empreinte digitale

Phosphorylcholine
Fibronectins
Coatings
Molecules
Fluorocarbon Polymers
Adsorption
Fluorocarbons
Depth profiling
Stainless Steel
Endothelial cells
Biocompatible Materials
Biomolecules
Secondary ion mass spectrometry
Biomaterials
Contact angle
Stainless steel
X ray photoelectron spectroscopy
Proteins
Plasmas
Imaging techniques

mots-clés

    Citer ceci

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    title = "Interaction of phosphorylcholine with fibronectin coatings: Surface characterization and biological performances",
    abstract = "Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.",
    keywords = "Biomaterials, Endothelialization, Fibronectin, Grafting, Hemocompatibility, Phosphorylcholine",
    author = "Vanessa Monta{\~n}o-Machado and C{\'e}line No{\"e}l and Pascale Chevallier and St{\'e}phane Turgeon and Laurent Houssiau and Emmanuel Pauthe and Jean-Jacques Pireaux and Diego Mantovani",
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    Interaction of phosphorylcholine with fibronectin coatings : Surface characterization and biological performances. / Montaño-Machado, Vanessa; Noël, Céline; Chevallier, Pascale; Turgeon, Stéphane; Houssiau, Laurent; Pauthe, Emmanuel; Pireaux, Jean-Jacques; Mantovani, Diego.

    Dans: Applied Surface Science, Vol 396, 28.02.2017, p. 1613-1622.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - Interaction of phosphorylcholine with fibronectin coatings

    T2 - Applied Surface Science

    AU - Montaño-Machado,Vanessa

    AU - Noël,Céline

    AU - Chevallier,Pascale

    AU - Turgeon,Stéphane

    AU - Houssiau,Laurent

    AU - Pauthe,Emmanuel

    AU - Pireaux,Jean-Jacques

    AU - Mantovani,Diego

    PY - 2017/2/28

    Y1 - 2017/2/28

    N2 - Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.

    AB - Coating medical devices with several bioactive molecules is an interesting approach to achieve specific biological targets upon the interaction of the biomaterial with the living environment. In this work, a fluorocarbon polymer (CFx) was first deposited by plasma treatment on stainless steel (SS) substrate and thereafter, coatings containing fibronectin (FN) and phosphorylcholine (PRC) were created for cardiovascular applications. These two biomolecules were chosen to promote endothelialization and to avoid thrombus formation, respectively. Adsorption and grafting techniques were applied – and combined – to accomplish 4 different coatings containing both molecules. However, big challenge was found to characterize a small molecule (PRC: 184 g/mol) interacting with a protein (FN: 450 kD). For the first time XPS, dynamic water contact angle, immunostaining and ToF-SIMS (imaging and depth profiling) analyses were combined to accomplish the characterization of such a coating. The most encouraging biological performances were obtained for samples where FN was grafted to the CFx film followed by the adsorption of PRC: proliferation of endothelial cells and hemocompatibility properties were observed. Promising coatings for cardiovascular applications were developed. The relevance of characterizing the coatings with high sensitive techniques and the further correlation with their biological performances were evidenced.

    KW - Biomaterials

    KW - Endothelialization

    KW - Fibronectin

    KW - Grafting

    KW - Hemocompatibility

    KW - Phosphorylcholine

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    M3 - Article

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    JO - Applied Surface Science

    JF - Applied Surface Science

    SN - 0169-4332

    ER -