TY - JOUR
T1 - Integrative multi-biomarker approach on caged rainbow trout
T2 - A biomonitoring tool for wastewater treatment plant effluents toxicity assessment
AU - Beghin, Mahaut
AU - Paris-Palacios, Séverine
AU - Mandiki, Syaghalirwa N.M.
AU - Schmitz, Mélodie
AU - Palluel, Olivier
AU - Gillet, Erin
AU - Bonnard, Isabelle
AU - Nott, Katherine
AU - Robert, Christelle
AU - Porcher, Jean Marc
AU - Ronkart, Sébastien
AU - Kestemont, Patrick
N1 - Funding Information:
This work carried out in the framework of the Project DIADEM (Développement d'une Approche Intégrée pour le Diagnostic de la qualité des Eaux de la Meuse) and was supported by the European Development Fund Interreg France-Wallonia-Vlaanderen (European Union) and the Public Service of Wallonia (SPW DGO3), as well as the FRIA (Fond de la Recherche dans l'Industrie et l'Agriculture, Walonia-Brussels Federation), providing a grant to M.B. We are grateful to the scientific and technical staff of the Research Unit in Environmental and Evolutionary Biology (URBE) of the University of Namur for their support during the caging setup and the fish organ sampling. The author thanks all the members of the DIADeM project who provided help for data collection and analysis.
Funding Information:
This work carried out in the framework of the Project DIADEM (Développement d’une Approche Intégrée pour le Diagnostic de la qualité des Eaux de la Meuse) and was supported by the European Development Fund Interreg France-Wallonia-Vlaanderen ( European Union ) and the Public Service of Wallonia (SPW DGO3), as well as the FRIA ( Fond de la Recherche dans l’Industrie et l’Agriculture , Walonia-Brussels Federation), providing a grant to M.B. We are grateful to the scientific and technical staff of the Research Unit in Environmental and Evolutionary Biology (URBE) of the University of Namur for their support during the caging setup and the fish organ sampling. The author thanks all the members of the DIADeM project who provided help for data collection and analysis.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/9/10
Y1 - 2022/9/10
N2 - The complex mixtures of contaminants released in wastewater treatment plant (WWTP) effluents are a major source of pollution for aquatic ecosystems. The present work aimed to assess the environmental risk posed by WWTP effluents by applying a multi-biomarker approach on caged rainbow trout (Oncorhynchus mykiss) juveniles. Fish were caged upstream and downstream of a WWTP for 21 days. To evaluate fish health, biomarkers representing immune, reproductive, nervous, detoxification, and antioxidant functions were assayed. Biomarker responses were then synthesized using an Integrated Biomarker Response (IBR) index. The IBR highlighted similar response patterns for the upstream and downstream sites. Caged juvenile females showed increased activities of innate immune parameters (lysozyme and complement), histological lesions and reduced glycogen content in the hepatic tissue, and higher muscle cholinergic metabolism. However, the intensity of the observed effects was more severe downstream of the WWTP. The present results suggest that the constitutive pollution level of the Meuse River measured upstream from the studied WWTP can have deleterious effects on fish health condition, which are exacerbated by the exposure to WWTP effluents. Our results infer that the application of IBR index is a promising tool to apply with active biomonitoring approaches as it provides comprehensive information about the biological effects caused by point source pollution such as WWTP, but also by the constitutive pollutions levels encountered in the receiving environment.
AB - The complex mixtures of contaminants released in wastewater treatment plant (WWTP) effluents are a major source of pollution for aquatic ecosystems. The present work aimed to assess the environmental risk posed by WWTP effluents by applying a multi-biomarker approach on caged rainbow trout (Oncorhynchus mykiss) juveniles. Fish were caged upstream and downstream of a WWTP for 21 days. To evaluate fish health, biomarkers representing immune, reproductive, nervous, detoxification, and antioxidant functions were assayed. Biomarker responses were then synthesized using an Integrated Biomarker Response (IBR) index. The IBR highlighted similar response patterns for the upstream and downstream sites. Caged juvenile females showed increased activities of innate immune parameters (lysozyme and complement), histological lesions and reduced glycogen content in the hepatic tissue, and higher muscle cholinergic metabolism. However, the intensity of the observed effects was more severe downstream of the WWTP. The present results suggest that the constitutive pollution level of the Meuse River measured upstream from the studied WWTP can have deleterious effects on fish health condition, which are exacerbated by the exposure to WWTP effluents. Our results infer that the application of IBR index is a promising tool to apply with active biomonitoring approaches as it provides comprehensive information about the biological effects caused by point source pollution such as WWTP, but also by the constitutive pollutions levels encountered in the receiving environment.
KW - Caging
KW - Integrated biomarker response
KW - Pharmaceuticals
KW - Wastewater treatment plant
UR - http://www.scopus.com/inward/record.url?scp=85130562252&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2022.155912
DO - 10.1016/j.scitotenv.2022.155912
M3 - Article
C2 - 35588819
AN - SCOPUS:85130562252
SN - 0048-9697
VL - 838
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 155912
ER -
