Influence of apixaban on commonly used coagulation assays: results from the Belgian national External Quality Assessment Scheme

Marjan van Blerk, Els Bailleul, B Chatelain, Anne Demulder, Katrien Devreese, J Douxfils, M Jacquemin, K Jochmans, F Mullier, Walter Wijns, B China, K Vernelen, M R Soumali

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

INTRODUCTION: The Belgian national External Quality Assessment Scheme performed a survey to assess the effect of the direct oral anticoagulant apixaban on the coagulation assays prothrombin time (PT), activated partial thromboplastin time (aPTT), fibrinogen and antithrombin as performed with a large number of reagent/instrument combinations.

METHODS: Four lyophilized plasma samples spiked with apixaban (0, 41, 94 and 225 ng/mL) were sent to the 195 Belgian and Luxembourg clinical laboratories performing coagulation testing.

RESULTS: PT and aPTT were barely influenced at the concentrations tested. At 225 ng/mL apixaban, PT and aPTT clotting times were only 1.15 times longer than at 0 ng/mL. Among PT reagents, RecombiPlasTin 2G(®) showed a slightly higher sensitivity with 225 ng/mL apixaban prolonging the PT clotting time 1.3-fold. Among aPTT reagents, there was no appreciable difference in sensitivity. Fibrinogen results were unaffected by the presence of apixaban, but antithrombin activity was considerably overestimated when measured with a FXa-based assay. At 225 ng/mL apixaban, the median percentage increase in antithrombin level was 31% when measured with the Liquid Antithrombin(®) reagent and 44% with the Innovance Antithrombin(®) reagent.

CONCLUSION: Our data provide clinical laboratories with useful information on the impact of apixaban on their routine coagulation assays.

langue originaleAnglais
Pages (de - à)402-408
Nombre de pages7
journalInternational Journal of Laboratory Hematology
Volume39
Numéro de publication4
Date de mise en ligne précoce2017
Les DOIs
Etat de la publicationPublié - 1 août 2017
Modification externeOui

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