Résumé
Cetuximab-conjugated gold nanoparticles are known to target cancer cells, but display toxicity towards normal kidney, liver and endothelial cells in vitro. In this study, we investigated their pharmacokinetics, biodistribution and toxicity after intravenous administration in healthy mice. Our data showed that these nanoparticles were rapidly cleared from the blood and accumulated mainly in the liver and spleen with long-term retention. Acute liver injury, inflammatory activity and vascular damage were transient and negligible, as confirmed by the liver functionality tests and serum marker analysis. There was no sign of altered liver, kidney, lung and spleen morphology up to 4 weeks post-injection. After 6 months, kidney casts and splenic apoptosis appeared to be more prevalent than in the controls. Furthermore, occasional immune cell infiltration was observed in the lungs. Therefore, we recommend additional in vivo studies, in order to investigate the long-term toxicity and elimination of gold nanoparticles after multiple dosing in their preclinical validation as new targeted anti-cancer therapies.
langue originale | Anglais |
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Pages (de - à) | 985-996 |
Nombre de pages | 12 |
journal | Journal of Biomedical Nanotechnology |
Volume | 16 |
Numéro de publication | 6 |
Les DOIs | |
Etat de la publication | Publié - 1 juin 2020 |
Empreinte digitale
Examiner les sujets de recherche de « In Vivo Pharmacokinetics, Biodistribution and Toxicity of Antibody-Conjugated Gold Nanoparticles in Healthy Mice ». Ensemble, ils forment une empreinte digitale unique.Thèses de l'étudiant
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Hybrid gold nanoparticles coated with organic polymers and antibodies as platforms for cancer theranostics: Investigation of the cytotoxicity mechanisms and feasibility study of radioactive labeling
DAEMS, N. (Auteur), Lucas, S. (Promoteur), MICHIELS, C. (Copromoteur), Aerts, A. (Copromoteur), MASEREEL, B. (Président), Van Hoecke, K. (Jury), Baatout, S. (Jury) & Millot, N. (Jury), 2 sept. 2020Student thesis: Doc types › Docteur en Sciences
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