Impact of lignans on the polyunsaturated fatty acid metabolic processing in a rainbow trout (Oncorhynchus mykiss) cell line

Julie Mellery, Florian Scalisi, Chloé Bonnineau, Patrick Kestemont, Xavier Rollin, Yvan Larondelle

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

In the context of fish oil replacement by alternative plant-derived oils in the fish feed, the influence of lignans, which are phenolic compounds present at significant concentrations in some plant-derived oils, was investigated on the omega-3 (n-3) fatty acid metabolism of the rainbow trout liver cell line RTL-W1 enriched in alpha-linolenic acid (ALA, 18:3n-3). The major linseed oil lignans, namely secoisolariciresinol diglucoside (SDG) and secoisolariciresinol (SECO), as well as the enterolignan enterodiol (END) were evaluated. Moreover, the effects of sesame oil lignans, namely sesamin (SES) and episesamin (EPI), were also investigated and compared to those previously observed in in vivo and in vitro studies which reported favourable effects on the fatty acid bioconversion capacity of salmonids. Cells were incubated with 50 μM ALA and 50 μM of SES, EPI, SDG, SECO or END for 48 h at 19 °C. The cells incubated with ALA had significantly higher amounts of 18:4n-3, 20:4n-3 and 20:5n-3 (eicosapentaenoic acid, EPA) as compared to control cells. The supplementation with SES decreased the 18:4n-3, 20:4n-3 and EPA contents, which indicates an inhibition of the desaturation and elongation steps involved in the fatty acid bioconversion pathway. As compared to SES supplementation, EPI supplementation showed a limited impact by only reducing 18:4n-3 and 20:4n-3. Similarly, END supplementation decreased 18:4n-3 and EPA, suggesting an inhibition of the desaturation capacity. Conversely, the supplementation of linseed lignans SDG and SECO had no impact on the fatty acid bioconversion capacity of RTL-W1 liver cells. In conclusion, the present results on the sesame seed lignans SES and EPI contradict the positive effects previously reported on fish lipid metabolism. In contrast, no influence of the linseed lignans SDG and SECO was observed. This suggests that the presence of lignans in linseed oil has no impact on the fish fatty acid bioconversion capacity when the oil is included to the diet.

langueAnglais
Pages106-110
Nombre de pages5
journalAquaculture
Volume476
Les DOIs
étatPublié - 1 juil. 2017

Empreinte digitale

lignans
rainbow
polyunsaturated fatty acids
Oncorhynchus mykiss
fatty acid
cell lines
biotransformation
oil
eicosapentaenoic acid
fatty acids
linseed oil
acid
linseed
fish
omega-3 fatty acids
oils
hepatocytes
metabolism
sesame seed
sesame oil

mots-clés

    Citer ceci

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    title = "Impact of lignans on the polyunsaturated fatty acid metabolic processing in a rainbow trout (Oncorhynchus mykiss) cell line",
    abstract = "In the context of fish oil replacement by alternative plant-derived oils in the fish feed, the influence of lignans, which are phenolic compounds present at significant concentrations in some plant-derived oils, was investigated on the omega-3 (n-3) fatty acid metabolism of the rainbow trout liver cell line RTL-W1 enriched in alpha-linolenic acid (ALA, 18:3n-3). The major linseed oil lignans, namely secoisolariciresinol diglucoside (SDG) and secoisolariciresinol (SECO), as well as the enterolignan enterodiol (END) were evaluated. Moreover, the effects of sesame oil lignans, namely sesamin (SES) and episesamin (EPI), were also investigated and compared to those previously observed in in vivo and in vitro studies which reported favourable effects on the fatty acid bioconversion capacity of salmonids. Cells were incubated with 50 μM ALA and 50 μM of SES, EPI, SDG, SECO or END for 48 h at 19 °C. The cells incubated with ALA had significantly higher amounts of 18:4n-3, 20:4n-3 and 20:5n-3 (eicosapentaenoic acid, EPA) as compared to control cells. The supplementation with SES decreased the 18:4n-3, 20:4n-3 and EPA contents, which indicates an inhibition of the desaturation and elongation steps involved in the fatty acid bioconversion pathway. As compared to SES supplementation, EPI supplementation showed a limited impact by only reducing 18:4n-3 and 20:4n-3. Similarly, END supplementation decreased 18:4n-3 and EPA, suggesting an inhibition of the desaturation capacity. Conversely, the supplementation of linseed lignans SDG and SECO had no impact on the fatty acid bioconversion capacity of RTL-W1 liver cells. In conclusion, the present results on the sesame seed lignans SES and EPI contradict the positive effects previously reported on fish lipid metabolism. In contrast, no influence of the linseed lignans SDG and SECO was observed. This suggests that the presence of lignans in linseed oil has no impact on the fish fatty acid bioconversion capacity when the oil is included to the diet.",
    keywords = "Enterolignans, Fatty acid metabolism, Linseed lignans, RTL-W1 cell line, Salmonids, Sesame seed lignans",
    author = "Julie Mellery and Florian Scalisi and Chlo{\'e} Bonnineau and Patrick Kestemont and Xavier Rollin and Yvan Larondelle",
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    journal = "Aquaculture",
    issn = "0044-8486",
    publisher = "Elsevier",

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    Impact of lignans on the polyunsaturated fatty acid metabolic processing in a rainbow trout (Oncorhynchus mykiss) cell line. / Mellery, Julie; Scalisi, Florian; Bonnineau, Chloé; Kestemont, Patrick; Rollin, Xavier; Larondelle, Yvan.

    Dans: Aquaculture, Vol 476, 01.07.2017, p. 106-110.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - Impact of lignans on the polyunsaturated fatty acid metabolic processing in a rainbow trout (Oncorhynchus mykiss) cell line

    AU - Mellery,Julie

    AU - Scalisi,Florian

    AU - Bonnineau,Chloé

    AU - Kestemont,Patrick

    AU - Rollin,Xavier

    AU - Larondelle,Yvan

    PY - 2017/7/1

    Y1 - 2017/7/1

    N2 - In the context of fish oil replacement by alternative plant-derived oils in the fish feed, the influence of lignans, which are phenolic compounds present at significant concentrations in some plant-derived oils, was investigated on the omega-3 (n-3) fatty acid metabolism of the rainbow trout liver cell line RTL-W1 enriched in alpha-linolenic acid (ALA, 18:3n-3). The major linseed oil lignans, namely secoisolariciresinol diglucoside (SDG) and secoisolariciresinol (SECO), as well as the enterolignan enterodiol (END) were evaluated. Moreover, the effects of sesame oil lignans, namely sesamin (SES) and episesamin (EPI), were also investigated and compared to those previously observed in in vivo and in vitro studies which reported favourable effects on the fatty acid bioconversion capacity of salmonids. Cells were incubated with 50 μM ALA and 50 μM of SES, EPI, SDG, SECO or END for 48 h at 19 °C. The cells incubated with ALA had significantly higher amounts of 18:4n-3, 20:4n-3 and 20:5n-3 (eicosapentaenoic acid, EPA) as compared to control cells. The supplementation with SES decreased the 18:4n-3, 20:4n-3 and EPA contents, which indicates an inhibition of the desaturation and elongation steps involved in the fatty acid bioconversion pathway. As compared to SES supplementation, EPI supplementation showed a limited impact by only reducing 18:4n-3 and 20:4n-3. Similarly, END supplementation decreased 18:4n-3 and EPA, suggesting an inhibition of the desaturation capacity. Conversely, the supplementation of linseed lignans SDG and SECO had no impact on the fatty acid bioconversion capacity of RTL-W1 liver cells. In conclusion, the present results on the sesame seed lignans SES and EPI contradict the positive effects previously reported on fish lipid metabolism. In contrast, no influence of the linseed lignans SDG and SECO was observed. This suggests that the presence of lignans in linseed oil has no impact on the fish fatty acid bioconversion capacity when the oil is included to the diet.

    AB - In the context of fish oil replacement by alternative plant-derived oils in the fish feed, the influence of lignans, which are phenolic compounds present at significant concentrations in some plant-derived oils, was investigated on the omega-3 (n-3) fatty acid metabolism of the rainbow trout liver cell line RTL-W1 enriched in alpha-linolenic acid (ALA, 18:3n-3). The major linseed oil lignans, namely secoisolariciresinol diglucoside (SDG) and secoisolariciresinol (SECO), as well as the enterolignan enterodiol (END) were evaluated. Moreover, the effects of sesame oil lignans, namely sesamin (SES) and episesamin (EPI), were also investigated and compared to those previously observed in in vivo and in vitro studies which reported favourable effects on the fatty acid bioconversion capacity of salmonids. Cells were incubated with 50 μM ALA and 50 μM of SES, EPI, SDG, SECO or END for 48 h at 19 °C. The cells incubated with ALA had significantly higher amounts of 18:4n-3, 20:4n-3 and 20:5n-3 (eicosapentaenoic acid, EPA) as compared to control cells. The supplementation with SES decreased the 18:4n-3, 20:4n-3 and EPA contents, which indicates an inhibition of the desaturation and elongation steps involved in the fatty acid bioconversion pathway. As compared to SES supplementation, EPI supplementation showed a limited impact by only reducing 18:4n-3 and 20:4n-3. Similarly, END supplementation decreased 18:4n-3 and EPA, suggesting an inhibition of the desaturation capacity. Conversely, the supplementation of linseed lignans SDG and SECO had no impact on the fatty acid bioconversion capacity of RTL-W1 liver cells. In conclusion, the present results on the sesame seed lignans SES and EPI contradict the positive effects previously reported on fish lipid metabolism. In contrast, no influence of the linseed lignans SDG and SECO was observed. This suggests that the presence of lignans in linseed oil has no impact on the fish fatty acid bioconversion capacity when the oil is included to the diet.

    KW - Enterolignans

    KW - Fatty acid metabolism

    KW - Linseed lignans

    KW - RTL-W1 cell line

    KW - Salmonids

    KW - Sesame seed lignans

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    U2 - 10.1016/j.aquaculture.2017.04.022

    DO - 10.1016/j.aquaculture.2017.04.022

    M3 - Article

    VL - 476

    SP - 106

    EP - 110

    JO - Aquaculture

    T2 - Aquaculture

    JF - Aquaculture

    SN - 0044-8486

    ER -