Identification of virulent Capnocytophaga canimorsus isolates by capsular typing

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Résumé

Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in the resistance to the innate immune system of the host. Here we developed the first C. canimorsus capsular serotyping scheme. We describe nine different serovars (A to I), which allowed to type 25/25 isolates from human infections but only 18/52 isolates from dog mouths, indicating that the repertoire of capsules in the species is vast. However, three serovars only (A, B, and C) covered 88 % of the human isolates tested (22/25) while they covered only 7.7 % of the dog isolates (4/52). Serovars A, B, and C were found 22.9, 14.6, and 4.2 fold respectively more often among human isolates than among dog isolates, with no geographical bias, implying that isolates endowed with these three capsular types are more virulent for humans than other isolates. Capsular serotyping would thus allow to identify virulent isolates in dogs, which could contribute to the prevention of these infections. To this end, we developed a PCR typing method based on the amplification of specific capsular genes.
langueAnglais
Nombre de pages34
journalJournal of clinical microbiology
Les DOIs
étatPublié - 2017

Empreinte digitale

Capnocytophaga
Dogs
Serotyping
Infection
Capsules
Polysaccharides
Mouth
Immune System
Polymerase Chain Reaction
Genes
Serogroup

Citer ceci

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title = "Identification of virulent Capnocytophaga canimorsus isolates by capsular typing",
abstract = "Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in the resistance to the innate immune system of the host. Here we developed the first C. canimorsus capsular serotyping scheme. We describe nine different serovars (A to I), which allowed to type 25/25 isolates from human infections but only 18/52 isolates from dog mouths, indicating that the repertoire of capsules in the species is vast. However, three serovars only (A, B, and C) covered 88 {\%} of the human isolates tested (22/25) while they covered only 7.7 {\%} of the dog isolates (4/52). Serovars A, B, and C were found 22.9, 14.6, and 4.2 fold respectively more often among human isolates than among dog isolates, with no geographical bias, implying that isolates endowed with these three capsular types are more virulent for humans than other isolates. Capsular serotyping would thus allow to identify virulent isolates in dogs, which could contribute to the prevention of these infections. To this end, we developed a PCR typing method based on the amplification of specific capsular genes.",
author = "Estelle Hess and Francesco Renzi and Dunia Koudad and M{\'e}lanie Dol and Guy Cornelis",
year = "2017",
doi = "10.1128/JCM.00249-17",
language = "English",
journal = "Journal of clinical microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",

}

TY - JOUR

T1 - Identification of virulent Capnocytophaga canimorsus isolates by capsular typing

AU - Hess, Estelle

AU - Renzi, Francesco

AU - Koudad, Dunia

AU - Dol, Mélanie

AU - Cornelis, Guy

PY - 2017

Y1 - 2017

N2 - Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in the resistance to the innate immune system of the host. Here we developed the first C. canimorsus capsular serotyping scheme. We describe nine different serovars (A to I), which allowed to type 25/25 isolates from human infections but only 18/52 isolates from dog mouths, indicating that the repertoire of capsules in the species is vast. However, three serovars only (A, B, and C) covered 88 % of the human isolates tested (22/25) while they covered only 7.7 % of the dog isolates (4/52). Serovars A, B, and C were found 22.9, 14.6, and 4.2 fold respectively more often among human isolates than among dog isolates, with no geographical bias, implying that isolates endowed with these three capsular types are more virulent for humans than other isolates. Capsular serotyping would thus allow to identify virulent isolates in dogs, which could contribute to the prevention of these infections. To this end, we developed a PCR typing method based on the amplification of specific capsular genes.

AB - Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in the resistance to the innate immune system of the host. Here we developed the first C. canimorsus capsular serotyping scheme. We describe nine different serovars (A to I), which allowed to type 25/25 isolates from human infections but only 18/52 isolates from dog mouths, indicating that the repertoire of capsules in the species is vast. However, three serovars only (A, B, and C) covered 88 % of the human isolates tested (22/25) while they covered only 7.7 % of the dog isolates (4/52). Serovars A, B, and C were found 22.9, 14.6, and 4.2 fold respectively more often among human isolates than among dog isolates, with no geographical bias, implying that isolates endowed with these three capsular types are more virulent for humans than other isolates. Capsular serotyping would thus allow to identify virulent isolates in dogs, which could contribute to the prevention of these infections. To this end, we developed a PCR typing method based on the amplification of specific capsular genes.

U2 - 10.1128/JCM.00249-17

DO - 10.1128/JCM.00249-17

M3 - Article

JO - Journal of clinical microbiology

T2 - Journal of clinical microbiology

JF - Journal of clinical microbiology

SN - 0095-1137

ER -