The outbreak of bovine spongiform encephalopathy (BSE) in the United Kingdom in 1986, with processed animal proteins (PAPs) as the main vector of the disease, has led to their prohibition in feed. The progressive release of the feed ban required the development of new analytical methods to determine the exact origin of PAPs from meat and bone meal. We set up a promising MS-based method to determine the species and the source (legal or not) present in PAPs: a TCA-acetone protein extraction followed by a cleanup step, an in-solution tryptic digestion of 5 h (with a 1:20 protein/trypsin ratio), and mass spectrometry analyses, first without any a priori, with a Q-TOF, followed by a targeted triple-quadrupole analysis. Using this procedure, we were able to overcome some of the major limitations of the official methods to analyze PAPs, detecting and identifying prohibited animal products in feedstuffs by the monitoring of peptides specific for cows, pigs, and sheep in PAPs.
Analyses peptidomiques pour la détection et l’identification des composants protéiques d’origine animale dans l’alimentation des animaux de renteAuthor: Marbaix, H., 25 mars 2016
Superviseur: Raes, M. (Promoteur), Mauro, S. (Personne externe) (Copromoteur), Arnould, T. (Président), Baeten, V. (Personne externe) (Jury), Rasinger, J. D. (Personne externe) (Jury), Vertommen, D. (Personne externe) (Jury) & Delahaut, P. (Personne externe) (Jury)
Thèse de l'étudiant: Doc types › Docteur en SciencesFichier