TY - JOUR
T1 - Identification of potential anti-photoageing algal compounds using an in-vitro model of photoageing
AU - Debacq-Chainiaux, Florence
AU - Borlon, C.
AU - De Hertogh, B.
AU - Remacle, José
AU - Morvan, P.-Y.
AU - Vallée, R.
AU - Toussaint, Olivier
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Stress-induced premature senescence (SIPS) has been proposed as an in-vitro model for testing the long-term effects of stressful events and to find molecules/natural extracts that protect against such stress. Premature senescence of human skin diploid fibroblasts (HDFs) can be induced by repeated subcytotoxic exposure to UVB, with the appearance of so-called biomarkers of senescence such as growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene overexpression and the common 4977-bp mitochondrial DNA deletion. This model of UVB-induced premature senescence has been acknowledged as a robust in-vitro model in photoageing research. In this study, the potential anti-photoageing effects of a series of algal extracts were tested. The appearance of the biomarkers of UVB-induced premature senescence of HDFs was studied with or without algal extracts. One algal extract was shown to be particularly protective against UVB-induced SIPS. The results obtained here reinforce the notion that UVB-induced premature senescence of HDFs can be used to screen potential anti-photoageing compounds. © 2006 The Authors.
AB - Stress-induced premature senescence (SIPS) has been proposed as an in-vitro model for testing the long-term effects of stressful events and to find molecules/natural extracts that protect against such stress. Premature senescence of human skin diploid fibroblasts (HDFs) can be induced by repeated subcytotoxic exposure to UVB, with the appearance of so-called biomarkers of senescence such as growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene overexpression and the common 4977-bp mitochondrial DNA deletion. This model of UVB-induced premature senescence has been acknowledged as a robust in-vitro model in photoageing research. In this study, the potential anti-photoageing effects of a series of algal extracts were tested. The appearance of the biomarkers of UVB-induced premature senescence of HDFs was studied with or without algal extracts. One algal extract was shown to be particularly protective against UVB-induced SIPS. The results obtained here reinforce the notion that UVB-induced premature senescence of HDFs can be used to screen potential anti-photoageing compounds. © 2006 The Authors.
UR - http://www.scopus.com/inward/record.url?scp=33846011431&partnerID=8YFLogxK
U2 - 10.1211/jpp.58.12.0003
DO - 10.1211/jpp.58.12.0003
M3 - Article
C2 - 17331320
VL - 58
SP - 1577
EP - 1583
JO - Journal of Pharmacy and Pharmacology
JF - Journal of Pharmacy and Pharmacology
IS - 12
ER -