Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

Stéphanie Zdanov; Florence Debacq-Chainiaux; José Remacle; Olivier Toussaint

doi:10.1016/j.febslet.2006.10.064

Identification of p38^MAPK-dependent genes with changed transcript abundance in H₂O₂-induced premature senescence of IMR-90 hTERT human fibroblasts

Stéphanie Zdanov, Florence Debacq-Chainiaux, José Remacle, Olivier Toussaint

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H₂O₂. We showed herein that p38^MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H₂O₂. Selective inhibition of p38^MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38^MAPK inhibitor and p38^MAPK small interferent RNA, we identified several p38^MAPK-target genes differentially expressed in H₂O₂-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

langue originale	Anglais
Pages (de - à)	6455-6463
Nombre de pages	9
journal	FEBS Letters
Volume	580
Numéro de publication	27
Les DOIs	https://doi.org/10.1016/j.febslet.2006.10.064
Etat de la publication	Publié - 27 nov. 2006

Accès au document

10.1016/j.febslet.2006.10.064

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Contient cette citation

@article{bfcf57561abd46a69807db8ffb389ec6,

title = "Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts",

abstract = "Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. {\textcopyright} 2006 Federation of European Biochemical Societies.",

keywords = "Fibroblasts, Gene expression, Oxidative stress, p38, Senescence, Telomerase",

author = "St{\'e}phanie Zdanov and Florence Debacq-Chainiaux and Jos{\'e} Remacle and Olivier Toussaint",

year = "2006",

month = nov,

day = "27",

doi = "10.1016/j.febslet.2006.10.064",

language = "English",

volume = "580",

pages = "6455--6463",

journal = "FEBS Letters",

publisher = "Elsevier",

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}

Identification of p38^MAPK-dependent genes with changed transcript abundance in H₂O₂-induced premature senescence of IMR-90 hTERT human fibroblasts. / Zdanov, Stéphanie; Debacq-Chainiaux, Florence ; Remacle, José et al.
Dans: FEBS Letters, Vol 580, Numéro 27, 27.11.2006, p. 6455-6463.

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

TY - JOUR

T1 - Identification of p38MAPK-dependent genes with changed transcript abundance in H2O2-induced premature senescence of IMR-90 hTERT human fibroblasts

AU - Zdanov, Stéphanie

AU - Debacq-Chainiaux, Florence

AU - Remacle, José

AU - Toussaint, Olivier

PY - 2006/11/27

Y1 - 2006/11/27

N2 - Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

AB - Premature senescence of IMR-90 human diploid fibroblasts expressing telomerase (hTERT) establishes after exposure to an acute sublethal concentration of H2O2. We showed herein that p38MAPK was phosphorylated after exposure of IMR-90 hTERT cells to H2O2. Selective inhibition of p38MAPK activity attenuated the increase in the proportion of cells positive for senescence associated β-galactosidase activity. We generated a low density DNA array to study gene expression profiles of 240 senescence-related genes. Using this array, p38MAPK inhibitor and p38MAPK small interferent RNA, we identified several p38MAPK-target genes differentially expressed in H2O2-stressed IMR-90 hTERT fibroblasts. © 2006 Federation of European Biochemical Societies.

KW - Fibroblasts

KW - Gene expression

KW - Oxidative stress

KW - p38

KW - Senescence

KW - Telomerase

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U2 - 10.1016/j.febslet.2006.10.064

DO - 10.1016/j.febslet.2006.10.064

M3 - Article

C2 - 17101135

VL - 580

SP - 6455

EP - 6463

JO - FEBS Letters

JF - FEBS Letters

IS - 27

ER -