Identification of a cytotoxic molecule in heat-modified citrus pectin

Lionel Leclere, Maude Fransolet, Pierre Cambier, Sandy El Bkassiny, Abdellatif Tikad, Marc Dieu, Stéphane P. Vincent, Pierre Van Cutsem, Carine Michiels

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Résumé

Modified forms of citrus pectin possess anticancer properties. However, their mechanism of action and the structural features involved remain unclear. Here, we showed that citrus pectin modified by heat treatment displayed cytotoxic effects in cancer cells. A fractionation approach was used aiming to identify active molecules. Dialysis and ethanol precipitation followed by HPLC analysis evidenced that most of the activity was related to molecules with molecular weight corresponding to low degree of polymerization oligogalacturonic acid. Heat-treatment of galacturonic acid also generated cytotoxic molecules. Furthermore, heat-modified galacturonic acid and heat-fragmented pectin contained the same molecule that induced cell death when isolated by HPLC separation. Mass spectrometry analyses revealed that 4,5-dihydroxy-2-cyclopenten-1-one was one cytotoxic molecule present in heat-treated pectin. Finally, we synthesized the enantiopure (4R,5R)-4,5-dihydroxy-2-cyclopenten-1-one and demonstrated that this molecule was cytotoxic and induced a similar pattern of apoptotic-like features than heat-modified pectin.

langue originaleAnglais
Pages (de - à)39-51
Nombre de pages13
journalCarbohydrate Polymers
Volume137
Les DOIs
Etat de la publicationPublié - 2016

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