Résumé
Hypoxia can activate the endothelium toward a pro-inflammatory phenotype and enhance leukocyte adhesion. This process is involved in pathological conditions such as vascular remodeling or ischemia-reperfusion injury. This study was aimed to obtain a global picture of the response of the endothelial cells to hypoxia with respect to inflammatory genes. To this purpose, we used a low density DNA microarray specifically designed to quantitate the expression of genes involved in the inflammatory pathways and a customized real-time PCR array. The expression of several pro-inflammatory genes known to be NF-kB target genes was decreased after the incubation of endothelial cells under hypoxia. In parallel, a decrease in the DNA binding activity of this transcription factor was observed. On the other hand, HIF-1 DNA binding activity was increased as well as the expression of several genes known to be regulated by this factor. Among them are several pro-inflammatory genes whose overexpression could account for the increase in leukocyte adhesion to the hypoxic endothelial cells. We concluded that hypoxia does not shift the endothelial cell phenotype to a more pro-inflammatory one probably because of a decrease in the expression of several cytokines. On the other hand, a clear response to hypoxia was observed with HIF-1 probably playing an important role in this process.
langue originale | Anglais |
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Pages (de - à) | 733-747 |
Nombre de pages | 15 |
journal | Experimental Cell Research |
Volume | 315 |
Numéro de publication | 5 |
Les DOIs | |
Etat de la publication | Publié - 2009 |
Empreinte digitale
Examiner les sujets de recherche de « Hypoxia regulates inflammatory gene expression in endothelial cells ». Ensemble, ils forment une empreinte digitale unique.Équipement
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Génétique - génomique
Gillet, N. (!!Manager)
Plateforme technologique Genetique et genomiqueEquipement/installations: Plateforme technolgique
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Morphologie - Imagerie
Cecchet, F. (!!Manager) & Renard, H.-F. (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Plateforme technolgique