TY - JOUR
T1 - Hybrid Alginate@TiO2 Porous Microcapsules as a Reservoir of Animal Cells for Cell Therapy
AU - Leroux, Grégory
AU - Neumann, Myriam
AU - Meunier, Christophe F.
AU - Fattaccioli, Antoine
AU - Michiels, Carine
AU - Arnould, Thierry
AU - Wang, Li
AU - Su, Bao Lian
N1 - Funding Information:
This work was supported by Program for Changjiang Scholars and Innovative Research Team in University (IRT_15R52) and “Algae Factory” (1610187) European H2020 program financed by FEDER and Wallonia Region of Belgium. B.-L.S. acknowledges the Chinese Central Government for an “Expert of the State” position in the Program of the “Thousand Talents” and the Chinese Ministry of Education for a “Changjiang Chair Professor” position. G.L. thanks the University of Namur for his assistant position to realize his PhD research. We are grateful to Dr. Marie-Ève Duprez of the University of Mons for the measurements of the Young’s modulus of the microcapsules. We thank Dr. Jun Jin and Prof. Yu Li from the State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of technology, Wuhan, China for their help.
Publisher Copyright:
© Copyright 2018 American Chemical Society.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018
Y1 - 2018
N2 - The number of patients suffering from diseases linked with hormone deficiency (e.g., type 1 diabetes mellitus) has significantly increased in recent years. As organ transplantation presents its limits, the design of novel robust devices for cell encapsulation is of great interest. The current study reports the design of a novel hybrid alginate microcapsule reinforced by titania via a biocompatible synthesis from an aqueous stable titania precursor (TiBALDH) and a cationic polyamine (PDDAC) under mild conditions. The biocompatibility of this one-pot synthesis was confirmed by evaluation of the cytotoxicity of the precursor, additive, product, and by-product. The morphology, structure, and properties of the obtained hybrid microcapsule were characterized in detail. The microcapsule displayed mesoporous, which was a key parameter to allow the diffusion of nutrients and metabolites and to avoid the entry of immune defenders. The hybrid microcapsule also showed enhanced mechanical stability compared to the pure alginate microcapsule, making it an ideal candidate as a cell reservoir. HepG2 model cells encapsulated in the hybrid microcapsules remained intact for 43 days as highlighted by fluorescent viability probes, their oxygen consumption, and their albumin secretion. The study provides a significant progress in the conception of the robust and biocompatible reservoirs of animal cells for cell therapy.
AB - The number of patients suffering from diseases linked with hormone deficiency (e.g., type 1 diabetes mellitus) has significantly increased in recent years. As organ transplantation presents its limits, the design of novel robust devices for cell encapsulation is of great interest. The current study reports the design of a novel hybrid alginate microcapsule reinforced by titania via a biocompatible synthesis from an aqueous stable titania precursor (TiBALDH) and a cationic polyamine (PDDAC) under mild conditions. The biocompatibility of this one-pot synthesis was confirmed by evaluation of the cytotoxicity of the precursor, additive, product, and by-product. The morphology, structure, and properties of the obtained hybrid microcapsule were characterized in detail. The microcapsule displayed mesoporous, which was a key parameter to allow the diffusion of nutrients and metabolites and to avoid the entry of immune defenders. The hybrid microcapsule also showed enhanced mechanical stability compared to the pure alginate microcapsule, making it an ideal candidate as a cell reservoir. HepG2 model cells encapsulated in the hybrid microcapsules remained intact for 43 days as highlighted by fluorescent viability probes, their oxygen consumption, and their albumin secretion. The study provides a significant progress in the conception of the robust and biocompatible reservoirs of animal cells for cell therapy.
KW - biocompatible synthesis
KW - cell encapsulation
KW - enhanced mechanical resistance
KW - hybrid hydrogel
KW - long-term cell activity
KW - porous nanostructure
UR - http://www.scopus.com/inward/record.url?scp=85055649595&partnerID=8YFLogxK
U2 - 10.1021/acsami.8b15483
DO - 10.1021/acsami.8b15483
M3 - Article
AN - SCOPUS:85055649595
SN - 1944-8244
VL - 10
SP - 37865
EP - 37877
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 44
ER -