Projets par an
Résumé
Brucella spp are intracellular bacteria that cause brucellosis, one of the most common zoonoses in the world. Given the serious medical consequences of this disease, a safe and effective human vaccine is urgently needed. Efforts to develop this vaccine have been hampered by our lack of understanding of what constitutes a protective memory response against Brucella. In this study, we characterize the cells and signaling pathways implicated in the generation of a protective immune memory response following priming by the injection of heat-killed or live Brucella melitensis 16M. Using a panel of gene-deficient mice, we demonstrated that during a secondary recall response, both the Brucella-specific humoral response and CD4(+) Th1 cells must act together to confer protective immunity in the spleen to B. melitensis infection. Humoral protective immunity is induced by the inoculation of both heat-killed and live bacteria, and its development does not require T cells, MyD88/IL-12p35 signaling pathways, or an activation-induced deaminase-mediated isotype switch. In striking contrast, the presence of memory IFN-γ-producing CD4(+) Th1 cells requires the administration of live bacteria and functional MyD88/IL-12p35 pathways. In summary, our work identifies several immune markers closely associated with protective immune memory and could help to define a rational strategy to obtain an effective human vaccine against brucellosis.
langue originale | Anglais |
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Pages (de - à) | 3740-52 |
Nombre de pages | 13 |
journal | Journal of immunology |
Volume | 192 |
Numéro de publication | 8 |
Les DOIs | |
Etat de la publication | Publié - 2014 |
Empreinte digitale Examiner les sujets de recherche de « Humoral Immunity and CD4+ Th1 Cells Are Both Necessary for a Fully Protective Immune Response upon Secondary Infection with Brucella melitensis ». Ensemble, ils forment une empreinte digitale unique.
Projets
- 1 Terminé
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Identifications in vivo des étapes du cycle infectieux de Brucella melitensis impliquées dans le développement d'une immunité protectrice chez la souris
LETESSON, J. & Hanot-Mambres, D.
1/10/10 → 30/09/12
Projet: Projet de thèse
Équipement
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FACS
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Equipement
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Morphologie - Imagerie
Charles Nicaise (!!Manager) & Francesca Cecchet (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Plateforme technolgique
Thèses de l'étudiant
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On the components of adaptive immune response involved in the control of a primary and secondary infection by Brucella melitensis
Author: Vitry, M., 15 mars 2013Superviseur: Letesson, J. (Promoteur), Muraille, É. (Copromoteur), Michiels, C. (Président), Leo, O. (Personne externe) (Jury), Goddeeris, B. (Personne externe) (Jury) & Baldwin, C. (Personne externe) (Jury)
Thèse de l'étudiant: Doc types › Docteur en Sciences