Hepatic accumulation and effects of microcystin-LR on juvenile goldfish Carassius auratus L.

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After intraperitoneal injection of microcystin-LR (MC-LR) (125 μg kg−1 body wt.), the concentration of MC-LR in the liver of juvenile goldfish Carassius auratus (30 g body wt.) was assayed by a modified protein phosphatase inhibition method. A temporary accumulation occurred from 3 to 48 h post-injection, followed by a significant decrease between 48 and 96 h. Under our experimental conditions, contamination by MC-LR did not change ionic homeostasis, as attested by blood osmolality values and gill Na+/K+ ATPase activity. Light microscopy observations revealed lesions and cellular necrosis progression, which was concomitant with an increase in enzyme activity of plasma aspartate aminotransferase (AspAT), alanine aminotransferase (AlaAT) and l-lactate dehydrogenase (LDH) and with a decrease of hepatic glutathione-S-transferase (GST) activity. Structural alterations and enzymatic activity modifications became significant within 24 h post-injection. Recovery of hepatocytes on day 21 after MC-LR injection was evident, together with a decrease in the MC-LR equivalent content of the liver.
langue originaleAnglais
Pages (de - à)39-48
Nombre de pages10
journalComparative Biochemistry and Physiology. Part C: Toxicology & Pharmacology
Numéro de publication1
Les DOIs
Etat de la publicationPublié - 2003

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