Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence

P. Dumont, V. Royer, T. Pascal, J.-F. Dierick, F. Chainiaux, C. Frippiat, J.P. De Magalhaes, F. Eliaers, J. Remacle, O. Toussaint

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM HO stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.
langue originaleAnglais
Pages (de - à)109-112
Nombre de pages4
journalFEBS Letters
Volume502
Numéro de publication3
Les DOIs
Etat de la publicationPublié - 3 août 2001

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