Functional assays in the diagnosis of heparin-induced thrombocytopenia: A review

Résultats de recherche: Contribution à un journal/une revueArticle de revue

Résumé

A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.

langueAnglais
Numéro d'article617
journalMolecules
Volume22
Numéro4
Les DOIs
étatPublié - 1 avr. 2017

Empreinte digitale

Thrombocytopenia
Heparin
Assays
Platelets
Blood Platelets
Chemical activation
Donor Selection
Platelet-Rich Plasma
Platelet Activation
Immunoassay
Plasmas
Suspensions
Technology
Standardization
Antibodies
Blood
Serum

mots-clés

    Citer ceci

    @article{903b9a0f901041afb837a59567693d73,
    title = "Functional assays in the diagnosis of heparin-induced thrombocytopenia: A review",
    abstract = "A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.",
    keywords = "Diagnosis, Functional assay, Heparin-induced thrombocytopenia, Platelets",
    author = "Valentine Minet and Dogn{\'e}, {Jean Michel} and Fran{\cc}ois Mullier",
    year = "2017",
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    day = "1",
    doi = "10.3390/molecules22040617",
    language = "English",
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    Functional assays in the diagnosis of heparin-induced thrombocytopenia : A review. / Minet, Valentine; Dogné, Jean Michel; Mullier, François.

    Dans: Molecules, Vol 22, Numéro 4, 617, 01.04.2017.

    Résultats de recherche: Contribution à un journal/une revueArticle de revue

    TY - JOUR

    T1 - Functional assays in the diagnosis of heparin-induced thrombocytopenia

    T2 - Molecules

    AU - Minet,Valentine

    AU - Dogné,Jean Michel

    AU - Mullier,François

    PY - 2017/4/1

    Y1 - 2017/4/1

    N2 - A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.

    AB - A rapid and accurate diagnosis in patients with suspected heparin-induced thrombocytopenia (HIT) is essential for patient management but remains challenging. Current HIT diagnosis ideally relies on a combination of clinical information, immunoassay and functional assay results. Platelet activation assays or functional assays detect HIT antibodies that are more clinically significant. Several functional assays have been developed and evaluated in the literature. They differ in the activation endpoint studied; the technique or technology used; the platelet donor selection; the platelet suspension (washed platelets, platelet rich plasma or whole blood); the patient sample (serum or plasma); and the heparin used (type and concentrations). Inconsistencies in controls performed and associated results interpretation are common. Thresholds and performances are determined differently among papers. Functional assays suffer from interlaboratory variability. This lack of standardization limits the evaluation and the accessibility of functional assays in laboratories. In the present article, we review all the current activation endpoints, techniques and methodologies of functional assays developed for HIT diagnosis.

    KW - Diagnosis

    KW - Functional assay

    KW - Heparin-induced thrombocytopenia

    KW - Platelets

    UR - http://www.scopus.com/inward/record.url?scp=85019655394&partnerID=8YFLogxK

    U2 - 10.3390/molecules22040617

    DO - 10.3390/molecules22040617

    M3 - Review article

    VL - 22

    JO - Molecules

    JF - Molecules

    SN - 1431-5165

    IS - 4

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    ER -