TY - JOUR
T1 - Feasibility of a mean platelet volume standard
T2 - an international council for standardization in hematology (ICSH) inter-laboratory study
AU - Harrison, Paul
AU - Didembourg, Marie
AU - Price, Joshua
AU - Johnson, Alan
AU - Baldwin, Samuel
AU - Veronneau, Marcel
AU - Baertlein, Daniel
AU - Shi, Xiaoyong
AU - Machin, Samuel
N1 - Funding Information:
The author(s) reported there is no funding associated with the work featured in this article. This paper is dedicated to the memory of Professor Samuel Machin who originally conceived the importance, concept and design of the project. This study was initiated and coordinated by an International Council for Standardization (ICSH) working group. The ICSH coordinates Working Groups of experts from around the globe to examine laboratory methods and instruments for hematological analyses, to deliberate on issues of standardization and to stimulate and coordinate scientific work as necessary toward the development of international standardization materials and guidelines. All standards used in this study were provided by Biotechne. Shipping costs were paid by the ICSH. The authors would like to thank all the participants for taking part in this study. Dr. Steven Marionneaux, Abbott Diagnostics, Santa Clara, CA, USA; Dr. Patrick O’Neil, Beckman Coulter, Miami, Florida, USA; Dr. Robert Langley, Siemens, Tarrytown, New York, USA; Dr. Yosuke Iwasaki, Sysmex Corporation, Kobe, Japan; Scott Lesher, Sysmex Corporation, Mundelein, IL, USA; Dr. Cécile Chabert, Horiba, Montpelier, France; Dr. Ertan Ergezen, Roche Diagnostics, Boston, MA, USA; Dr Albert Huisman, Utrecht, The Netherlands; Dr. Vicki Parsons, Kansas City, Kansas, USA; Dr. Cristian Morales, Barcelona, Spain; Ms. Cordula Sternemann, Bochum, Germany; Professor Marie-Christine Alessi, Marseille, France; Conor O’Malley, Leeds, UK; Dr. Paul Harrison, Birmingham, UK; Dr. Ian Mackie, London, UK; Dr. Sukesh C Nair, Vellore, Tamil Nadu, India; Dr Henk Russcher, Rotterdam, The Netherlands; Dr. Patrick Chan, Hong Kong, China and Dr. JianBiao Wang, Shanghai, China.
Publisher Copyright:
© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.
PY - 2022
Y1 - 2022
N2 - We have evaluated a commercial-fixed porcine platelet preparation (with and without added fixed human red blood cells (RBC)) for the potential standardization of mean platelet volume (MPV) measurements. The standards (Biotechne) were distributed internationally to 19 laboratories including all major hematology instrument manufacturers and academic/pathology laboratories. Overall, the standards demonstrated excellent stability up to 1 month within both MPV values and platelet counts when stored at 4°C. The presence of RBC significantly increased the platelet count and MPV values compared to platelets alone. However, as expected, there were differences in MPV values between different instruments and manufacturers. MPV values were also significantly higher in the whole blood standard compared to the platelet standard in the majority of instruments except with some instruments, where MPV values were significantly higher in the platelet only preparation. To further investigate this phenomenon, two different Platelet MPV preparations (with low and high MPV) in combination with 3 different RBC MCV preparations (with low, normal or high MCVs) were tested to try and further elucidate how RBC populations may impact upon platelet analysis (count, MPV, and PDW) using a single impedance analyzer. Both MPV and MCV values showed good stability over the course of the study for up to 50 days. As expected, the RBC preparation with the lowest MCV had the greatest impact on the MPV. However, this was not observed with an increase in MCV of the RBC or by a larger MPV of the platelet population. To further understand how different gating strategies may also influence results, we investigated the effect of either fixed or floating gate strategies upon MPV raw data from patient samples in a single impedance analyzer. Overall, it was clear that floating and fixed gate strategies also significantly impact upon MPV values. In conclusion, we have demonstrated the potential of an MPV standard with good stability characteristics for calibrating and comparing full blood counters that use different analysis principles, gating and MPV calculations. This may facilitate future instrument calibration and harmonization of results between different technologies.
AB - We have evaluated a commercial-fixed porcine platelet preparation (with and without added fixed human red blood cells (RBC)) for the potential standardization of mean platelet volume (MPV) measurements. The standards (Biotechne) were distributed internationally to 19 laboratories including all major hematology instrument manufacturers and academic/pathology laboratories. Overall, the standards demonstrated excellent stability up to 1 month within both MPV values and platelet counts when stored at 4°C. The presence of RBC significantly increased the platelet count and MPV values compared to platelets alone. However, as expected, there were differences in MPV values between different instruments and manufacturers. MPV values were also significantly higher in the whole blood standard compared to the platelet standard in the majority of instruments except with some instruments, where MPV values were significantly higher in the platelet only preparation. To further investigate this phenomenon, two different Platelet MPV preparations (with low and high MPV) in combination with 3 different RBC MCV preparations (with low, normal or high MCVs) were tested to try and further elucidate how RBC populations may impact upon platelet analysis (count, MPV, and PDW) using a single impedance analyzer. Both MPV and MCV values showed good stability over the course of the study for up to 50 days. As expected, the RBC preparation with the lowest MCV had the greatest impact on the MPV. However, this was not observed with an increase in MCV of the RBC or by a larger MPV of the platelet population. To further understand how different gating strategies may also influence results, we investigated the effect of either fixed or floating gate strategies upon MPV raw data from patient samples in a single impedance analyzer. Overall, it was clear that floating and fixed gate strategies also significantly impact upon MPV values. In conclusion, we have demonstrated the potential of an MPV standard with good stability characteristics for calibrating and comparing full blood counters that use different analysis principles, gating and MPV calculations. This may facilitate future instrument calibration and harmonization of results between different technologies.
KW - Mean cell volume
KW - mean platelet volume
KW - platelet count
KW - platelets
KW - standardization
UR - http://www.scopus.com/inward/record.url?scp=85129859151&partnerID=8YFLogxK
U2 - 10.1080/09537104.2022.2060956
DO - 10.1080/09537104.2022.2060956
M3 - Article
SN - 0953-7104
VL - 33
SP - 1159
EP - 1167
JO - Platelets
JF - Platelets
IS - 8
ER -