Fast Targeting and Cancer Cell Uptake of Luminescent Antibody-Nanozeolite Bioconjugates

Riccardo Marega, Eko Adi Prasetyanto, Carine Michiels, Luisa De Cola, Davide Bonifazi

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Understanding the targeted cellular uptake of nanomaterials is an essential step to engineer and program functional and effective biomedical devices. In this respect, the targeting and ultrafast uptake of zeolite nanocrystals functionalized with Cetuximab antibodies (Ctxb) by cells overexpressing the epidermal growth factor receptor are described here. Biochemical assays show that the cellular uptake of the bioconjugate in the targeted cancer cells already begins 15 min after incubation, at a rate around tenfold faster than that observed in the negative control cells. These findings further show the role of Ctxb exposed at the surfaces of the zeolite nanocrystals in mediating the targeted and rapid cellular uptake. By using temperature and pharmacological inhibitors as modulators of the internalization pathways, the results univocally suggest a dissipative uptake mechanism of these nanomaterials, which seems to occur using different internalization pathways, according to the targeting properties of these nanocrystals. Owing to the ultrafast uptake process, harmless for the cell viability, these results further pave the way for the design of novel theranostic tools based on nanozeolites.

langue originaleAnglais
Pages (de - à)5431-5441
Nombre de pages11
journalSmall
Les DOIs
étatPublié - 2016

Empreinte digitale

Antibodies
Nanoparticles
Nanocrystals
Zeolites
Nanostructures
Cells
Nanostructured materials
Neoplasms
Epidermal Growth Factor Receptor
Modulators
Assays
Cell Survival
Pharmacology
Engineers
Equipment and Supplies
Temperature
Cetuximab

Citer ceci

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title = "Fast Targeting and Cancer Cell Uptake of Luminescent Antibody-Nanozeolite Bioconjugates",
abstract = "Understanding the targeted cellular uptake of nanomaterials is an essential step to engineer and program functional and effective biomedical devices. In this respect, the targeting and ultrafast uptake of zeolite nanocrystals functionalized with Cetuximab antibodies (Ctxb) by cells overexpressing the epidermal growth factor receptor are described here. Biochemical assays show that the cellular uptake of the bioconjugate in the targeted cancer cells already begins 15 min after incubation, at a rate around tenfold faster than that observed in the negative control cells. These findings further show the role of Ctxb exposed at the surfaces of the zeolite nanocrystals in mediating the targeted and rapid cellular uptake. By using temperature and pharmacological inhibitors as modulators of the internalization pathways, the results univocally suggest a dissipative uptake mechanism of these nanomaterials, which seems to occur using different internalization pathways, according to the targeting properties of these nanocrystals. Owing to the ultrafast uptake process, harmless for the cell viability, these results further pave the way for the design of novel theranostic tools based on nanozeolites.",
keywords = "Cellular uptake, Cetuximab, Nanomaterials, Targeting, Zeolites",
author = "Riccardo Marega and Prasetyanto, {Eko Adi} and Carine Michiels and {De Cola}, Luisa and Davide Bonifazi",
year = "2016",
doi = "10.1002/smll.201601447",
language = "English",
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journal = "Small",
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Fast Targeting and Cancer Cell Uptake of Luminescent Antibody-Nanozeolite Bioconjugates. / Marega, Riccardo; Prasetyanto, Eko Adi; Michiels, Carine; De Cola, Luisa; Bonifazi, Davide.

Dans: Small, 2016, p. 5431-5441.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - Fast Targeting and Cancer Cell Uptake of Luminescent Antibody-Nanozeolite Bioconjugates

AU - Marega, Riccardo

AU - Prasetyanto, Eko Adi

AU - Michiels, Carine

AU - De Cola, Luisa

AU - Bonifazi, Davide

PY - 2016

Y1 - 2016

N2 - Understanding the targeted cellular uptake of nanomaterials is an essential step to engineer and program functional and effective biomedical devices. In this respect, the targeting and ultrafast uptake of zeolite nanocrystals functionalized with Cetuximab antibodies (Ctxb) by cells overexpressing the epidermal growth factor receptor are described here. Biochemical assays show that the cellular uptake of the bioconjugate in the targeted cancer cells already begins 15 min after incubation, at a rate around tenfold faster than that observed in the negative control cells. These findings further show the role of Ctxb exposed at the surfaces of the zeolite nanocrystals in mediating the targeted and rapid cellular uptake. By using temperature and pharmacological inhibitors as modulators of the internalization pathways, the results univocally suggest a dissipative uptake mechanism of these nanomaterials, which seems to occur using different internalization pathways, according to the targeting properties of these nanocrystals. Owing to the ultrafast uptake process, harmless for the cell viability, these results further pave the way for the design of novel theranostic tools based on nanozeolites.

AB - Understanding the targeted cellular uptake of nanomaterials is an essential step to engineer and program functional and effective biomedical devices. In this respect, the targeting and ultrafast uptake of zeolite nanocrystals functionalized with Cetuximab antibodies (Ctxb) by cells overexpressing the epidermal growth factor receptor are described here. Biochemical assays show that the cellular uptake of the bioconjugate in the targeted cancer cells already begins 15 min after incubation, at a rate around tenfold faster than that observed in the negative control cells. These findings further show the role of Ctxb exposed at the surfaces of the zeolite nanocrystals in mediating the targeted and rapid cellular uptake. By using temperature and pharmacological inhibitors as modulators of the internalization pathways, the results univocally suggest a dissipative uptake mechanism of these nanomaterials, which seems to occur using different internalization pathways, according to the targeting properties of these nanocrystals. Owing to the ultrafast uptake process, harmless for the cell viability, these results further pave the way for the design of novel theranostic tools based on nanozeolites.

KW - Cellular uptake

KW - Cetuximab

KW - Nanomaterials

KW - Targeting

KW - Zeolites

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