Résumé
Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.
langue originale | Anglais |
---|---|
Pages (de - à) | 2077-2082 |
Nombre de pages | 6 |
journal | Proteomics |
Volume | 13 |
Numéro de publication | 14 |
Les DOIs | |
Etat de la publication | Publié - 1 juil. 2013 |
Empreinte digitale
Examiner les sujets de recherche de « Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes ». Ensemble, ils forment une empreinte digitale unique.Équipement
-
Spectrométrie de masse (MASUN)
Patricia Renard (!!Manager)
Plateforme technologique Proteomique et spectrometrie de masseEquipement/installations: Plateforme technolgique