ERK activation upon hypoxia: involvement in HIF-1 activation

Emmanuel Minet, Thierry Arnould, Gaetan Michel, Isabelle Roland, Denis Mottet, Martine Raes, José Remacle, Carine Michiels

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

Hypoxia-inducible factor-1 (HIF-1) is a transcription factor activated by hypoxia. The HIF-1 activation transduction pathway is poorly understood. In this report, we investigated the activation of extracellular regulated kinases (ERK) in hypoxia and their involvement in HIF-1 activation. We demonstrated that in human microvascular endothelial cells-1 (HMEC-1), ERK kinases are activated during hypoxia. Using dominant negative mutants, we showed that ERK1 is needed for hypoxia-induced HIF-1 transactivation activity. Moreover, using a kinase assay and Western blot experiments, we showed that HIF-1α is phosphorylated in hypoxia by an ERK-dependent pathway. These results evidence the role of mitogen-activated protein kinase in the transcriptional response to hypoxia. Copyright (C) 2000 Federation of European Biochemical Societies.

langue originaleAnglais
Pages (de - à)53-58
Nombre de pages6
journalFEBS Letters
Volume468
Numéro de publication1
Les DOIs
Etat de la publicationPublié - 18 févr. 2000

Empreinte digitale

Examiner les sujets de recherche de « ERK activation upon hypoxia: involvement in HIF-1 activation ». Ensemble, ils forment une empreinte digitale unique.

Contient cette citation