TY - JOUR
T1 - Endocrine and scavenging cell functions of a juvenile fish heart atrium
T2 - Fine structure aspects and translating conjectures
AU - Gilloteaux, Jacques
AU - Jennes, Lothar
AU - Vanderhaeghen, Jean Jacques
N1 - Funding Information:
This study and data collection were in part supported by the Fonds National de la Recherche Scientifique et Médicale de Belgique while JG was in sabbatical leave invited by JJV Department of Neuropathology and Electron Microscopy, Université Libre de Bruxelles, Campus Erasme, Brussels (Belgium). LHJ provided ANF antibodies, other technical assistance came from Ms. Roberte Menu while the TR Kelly fund of Summa Health Research Foundation, Akron OH, USA, an award granted by the 1986 Ohio Board of Regents Research Challenge Fund. Northeastern Ohio Universities College of Medicine. The American Heart Akron Ohio Chapter helped to contribute to other parts of this study. JG wrote this report while Emeritus Professor of St George's University School of Medicine, Newcastle upon Tyne, United Kingdom, and Scientific Collaborator at URPHyM, Narilis, Université de Namur, Namur, Belgium.This study and data collection were in part supported by the Fonds National de la Recherche Scientifique et Médicale de Belgique while JG was in sabbatical leave invited by JJV Department of Neuropathology and Electron Microscopy, Université Libre de Bruxelles, Campus Erasme, Brussels (Belgium). LHJ provided ANF antibodies, other technical assistance came from Ms. Roberte Menu while the T.R. Kelly fund of Summa Health Research Foundation, Akron OH, USA, an award granted by the 1986 Ohio Board of Regents Research Challenge Fund. Northeastern Ohio Universities College of Medicine. The American Heart Akron Ohio Chapter helped to contribute to other parts of this study. JG wrote this report while Emeritus Professor of St George's University School of Medicine, Newcastle upon Tyne, United Kingdom, and Scientific Collaborator at URPHyM, Narilis, Université de Namur, Namur, Belgium.
Funding Information:
This study and data collection were in part supported by the Fonds National de la Recherche Scientifique et Médicale de Belgique while JG was in sabbatical leave invited by JJV Department of Neuropathology and Electron Microscopy, Université Libre de Bruxelles, Campus Erasme, Brussels (Belgium). LHJ provided ANF antibodies, other technical assistance came from Ms. Roberte Menu while the T.R. Kelly fund of Summa Health Research Foundation , Akron OH , USA , an award granted by the 1986 Ohio Board of Regents Research Challenge Fund. Northeastern Ohio Universities College of Medicine. The American Heart Akron Ohio Chapter helped to contribute to other parts of this study. JG wrote this report while Emeritus Professor of St George's University School of Medicine, Newcastle upon Tyne, United Kingdom, and Scientific Collaborator at URPHyM, Narilis, Université de Namur, Namur, Belgium.
Funding Information:
This study and data collection were in part supported by the Fonds National de la Recherche Scientifique et Médicale de Belgique while JG was in sabbatical leave invited by JJV Department of Neuropathology and Electron Microscopy, Université Libre de Bruxelles, Campus Erasme, Brussels (Belgium). LHJ provided ANF antibodies, other technical assistance came from Ms. Roberte Menu while the TR Kelly fund of Summa Health Research Foundation , Akron OH , USA , an award granted by the 1986 Ohio Board of Regents Research Challenge Fund. Northeastern Ohio Universities College of Medicine. The American Heart Akron Ohio Chapter helped to contribute to other parts of this study. JG wrote this report while Emeritus Professor of St George's University School of Medicine, Newcastle upon Tyne, United Kingdom, and Scientific Collaborator at URPHyM, Narilis, Université de Namur, Namur, Belgium.
Publisher Copyright:
© 2023 The Author(s)
PY - 2023/9
Y1 - 2023/9
N2 - Background: The atrium of the juvenile teleost, smallmouth bass, Micropterus dolomieu Lacépède showed a pigmented endocardium. Our goal was to use fine structure to survey this fish heart tissues and verify the content responsible for this shrouding and discuss mammalian, translational conjectures about this cardiac structure. Methods: Using electron microscopy, heart pericardium, myocardium, and endocardium were analyzed, including atrial peptide immunolabeling. Results: The endocardium endothelial cells of the atrium revealed pinocytosis and endocytosis activities that resulted in accumulated electron-contrasted secondary lysosomes and lipofuscin bodies not found in the ventricle endocardium. This endothelium contacted subjacent atrial and ventricular myocardial cells producing immunolabeled atrial peptide-containing vesicles. Other migrating cells, including melano-macrophages, were noticed in the atrial sub endocardium. Conclusions: The endocardium functioned as a potent blood-heart barrier where transcytosis and dispatching of numerous materials, including those atrial peptides, between myocardium and the circulation occur. Peculiarly, this juvenile endothelium revealed abundant lysosomal processing of scavenged circulation materials like in a reticuloendothelial tissue. These juvenile cell's accumulated ‘aging’ lipofuscin bodies as organized deposits into a network with other organelles, especially mitochondria without evident disposal. Additionally, other cells, including melano-macrophages roamed both sub endocardium and ventricle; those also can influence the viewed pigmentation. Some of these data comforted the endocardial mesodermal lineage, still sketchy for all vertebrates while fish heart development is still used.
AB - Background: The atrium of the juvenile teleost, smallmouth bass, Micropterus dolomieu Lacépède showed a pigmented endocardium. Our goal was to use fine structure to survey this fish heart tissues and verify the content responsible for this shrouding and discuss mammalian, translational conjectures about this cardiac structure. Methods: Using electron microscopy, heart pericardium, myocardium, and endocardium were analyzed, including atrial peptide immunolabeling. Results: The endocardium endothelial cells of the atrium revealed pinocytosis and endocytosis activities that resulted in accumulated electron-contrasted secondary lysosomes and lipofuscin bodies not found in the ventricle endocardium. This endothelium contacted subjacent atrial and ventricular myocardial cells producing immunolabeled atrial peptide-containing vesicles. Other migrating cells, including melano-macrophages, were noticed in the atrial sub endocardium. Conclusions: The endocardium functioned as a potent blood-heart barrier where transcytosis and dispatching of numerous materials, including those atrial peptides, between myocardium and the circulation occur. Peculiarly, this juvenile endothelium revealed abundant lysosomal processing of scavenged circulation materials like in a reticuloendothelial tissue. These juvenile cell's accumulated ‘aging’ lipofuscin bodies as organized deposits into a network with other organelles, especially mitochondria without evident disposal. Additionally, other cells, including melano-macrophages roamed both sub endocardium and ventricle; those also can influence the viewed pigmentation. Some of these data comforted the endocardial mesodermal lineage, still sketchy for all vertebrates while fish heart development is still used.
KW - Atrial natriuretic peptide
KW - Endocardium
KW - Endocytosis
KW - Fish
KW - Lipofuscin
KW - Melano-macrophage cell
KW - Pinocytosis
KW - Reticuloendothelial tissue
UR - http://www.scopus.com/inward/record.url?scp=85166322706&partnerID=8YFLogxK
U2 - 10.1016/j.tria.2023.100252
DO - 10.1016/j.tria.2023.100252
M3 - Article
AN - SCOPUS:85166322706
SN - 2214-854X
VL - 32
JO - Translational Research in Anatomy
JF - Translational Research in Anatomy
M1 - 100252
ER -