Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy

Laurent Dreesen, Christophe Humbert, Yannick Sartenaer, Yves Caudano, Cedric Volcke, Alaa Addin Mani, André Peremans, Paul Thiry, Sophie Hanique, Jean-Marie Frère

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Résumé

Two-color sum-frequency generation spectroscopy (2C-SFG) is used to probe the molecular and electronic properties of an adsorbed layer of the green fluorescent protein mutant 2 (GFPmut2) on a platinum (111) substrate. First, the spectroscopic measurements, performed under different polarization combinations, and atomic force microscopy (AFM) show that the GFPmut2 proteins form a fairly ordered monolayer on the platinum surface. Next, the nonlinear spectroscopic data provide evidence of particular coupling phenomena between the GFPmut2 vibrational and electronic properties. This is revealed by the occurrence of two doubly resonant sum-frequency generation processes for molecules having both their Raman and infrared transition moments in a direction perpendicular to the sample plane. Finally, our 2C-SFG analysis reveals two electronic transitions corresponding to the absorption and fluorescence energy levels which are related to two different GFPmut2 conformations: the B (anionic) and I forms, respectively. Their observation and wavelength positions attest the keeping of the GFPmut2 electronic properties upon adsorption on the metallic surface.
langue originaleAnglais
Pages (de - à)7201-7207
Nombre de pages7
journalLangmuir
Volume20
Numéro de publication17
Etat de la publicationPublié - 2004

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    Dreesen, L., Humbert, C., Sartenaer, Y., Caudano, Y., Volcke, C., Mani, A. A., Peremans, A., Thiry, P., Hanique, S., & Frère, J-M. (2004). Electronic and molecular properties of an adsorbed protein monolayer probed by two-color sum-frequency generation spectroscopy. Langmuir, 20(17), 7201-7207. http://dx.doi.org/10.1021/la0488001