TY - JOUR
T1 - Effect of procaine on cultivated human WI-38 fibroblasts
AU - Pigeolet, E.
AU - Raes, Martine
AU - Houbion, A.
AU - Remacle, J.
N1 - Medline is the source for the MeSH terms of this document.
PY - 1988/1/1
Y1 - 1988/1/1
N2 - Procaine is a local anesthetic, also used in experimental gerontology and has been tested in cultivated human WI-38 fibroblasts. This molecule was found to enhance growth rate and cell densities in actively dividing cultures. As the cells aged, however, this stimulatory effect diminished and finally vanished. In a long term experiment the enhancement of growth of procaine treated cultures was finally replaced by a toxic effect even at low concentration. The amount of the thermolabile enzyme found in phase III cells did not change when procaine was added to the culture medium. In this cellular aging model, procaine behaved like a metabolic stimulator of actively dividing cells but not as an 'antiaging' molecule as it is sometimes assumed.
AB - Procaine is a local anesthetic, also used in experimental gerontology and has been tested in cultivated human WI-38 fibroblasts. This molecule was found to enhance growth rate and cell densities in actively dividing cultures. As the cells aged, however, this stimulatory effect diminished and finally vanished. In a long term experiment the enhancement of growth of procaine treated cultures was finally replaced by a toxic effect even at low concentration. The amount of the thermolabile enzyme found in phase III cells did not change when procaine was added to the culture medium. In this cellular aging model, procaine behaved like a metabolic stimulator of actively dividing cells but not as an 'antiaging' molecule as it is sometimes assumed.
UR - http://www.scopus.com/inward/record.url?scp=0023916997&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0023916997
SN - 0531-5565
VL - 23
SP - 87
EP - 96
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 2
ER -