Deletions and an inversion induced by a resident IS1 of the lactose transposon Tn951

G Cornelis, H Saedler

    Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

    Résumé

    DNA-DNA filter binding tests, "Southern" blotting experiments and DNA heteroduplex analysis clearly show that Tn951 contains an IS1 element. This IS1-951 sequence is peculiar in that it does not contain the PstI cleavage site which is usually observed on E. coli derived IS1 elements. Nonetheless, IS1-951 induces deletions. This process is temperature dependent. One instance of an IS1-951 induced inversion was observed, the structure of which is compatible with the current models of transposition of IS elements.
    langue originaleAnglais
    Pages (de - à)367-74
    Nombre de pages8
    journalMolecular & general genetics : MGG
    Volume178
    Numéro de publication2
    Etat de la publicationPublié - 1980

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