CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus

Nayla Francis, Katy Poncin, Antonella Fioravanti, Victoria Vassen, Kevin Willemart, Thi Anh Phuong Ong, Luca Rappez, Jean-Jacques Letesson, Emanuele G Biondi, Xavier De Bolle

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Brucella abortus is a pathogen infecting cattle, able to survive, traffic, and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic, and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution.

langue originaleAnglais
Pages (de - à)780-797
Nombre de pages18
journalMolecular Microbiology
Numéro de publication5
Date de mise en ligne précoce2016
Les DOIs
Etat de la publicationPublié - mars 2017

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