CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution. This article is protected by copyright. All rights reserved.

langueAnglais
Pages780-797
Nombre de pages17
journalMolecular microbiology
Volume103
Numéro5
Date de mise en ligne précoce2016
Les DOIs
étatPublié - 2017

Empreinte digitale

Brucella abortus
Cell Division
Membranes
Bacteria
Alphaproteobacteria
Regulon
Cell Cycle
Genes
Caulobacter crescentus
HeLa Cells
Endoplasmic Reticulum
Lipopolysaccharides
Life Style
Membrane Proteins

Citer ceci

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title = "CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus",
abstract = "Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution. This article is protected by copyright. All rights reserved.",
author = "Nayla Francis and Katy Poncin and Antonella Fioravanti and Victoria Vassen and Kevin Willemart and Ong, {Thi Anh Phuong} and Luca Rappez and Jean-Jacques Letesson and Biondi, {Emanuele G} and {De Bolle}, Xavier",
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CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus. / Francis, Nayla; Poncin, Katy; Fioravanti, Antonella; Vassen, Victoria; Willemart, Kevin; Ong, Thi Anh Phuong; Rappez, Luca; Letesson, Jean-Jacques; Biondi, Emanuele G; De Bolle, Xavier.

Dans: Molecular microbiology, Vol 103, Numéro 5, 2017, p. 780-797.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus

AU - Francis,Nayla

AU - Poncin,Katy

AU - Fioravanti,Antonella

AU - Vassen,Victoria

AU - Willemart,Kevin

AU - Ong,Thi Anh Phuong

AU - Rappez,Luca

AU - Letesson,Jean-Jacques

AU - Biondi,Emanuele G

AU - De Bolle,Xavier

N1 - © 2016 John Wiley & Sons Ltd.

PY - 2017

Y1 - 2017

N2 - Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution. This article is protected by copyright. All rights reserved.

AB - Brucella abortus is a pathogen infecting cattle, able to survive, traffic and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution. This article is protected by copyright. All rights reserved.

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DO - 10.1111/mmi.13589

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SP - 780

EP - 797

JO - Molecular microbiology

T2 - Molecular microbiology

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SN - 0950-382X

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