Projets par an
Résumé
Brucella abortus is a pathogen infecting cattle, able to survive, traffic, and proliferate inside host cells. It belongs to the Alphaproteobacteria, a phylogenetic group comprising bacteria with free living, symbiotic, and pathogenic lifestyles. An essential regulator of cell cycle progression named CtrA was described in the model bacterium Caulobacter crescentus. This regulator is conserved in many alphaproteobacteria, but the evolution of its regulon remains elusive. Here we identified promoters that are CtrA targets using ChIP-seq and we found that CtrA binds to promoters of genes involved in cell cycle progression, in addition to numerous genes encoding outer membrane components involved in export of membrane proteins and synthesis of lipopolysaccharide. Analysis of a conditional B. abortus ctrA loss of function mutant confirmed that CtrA controls cell division. Impairment of cell division generates elongated and branched morphologies, that are also detectable inside HeLa cells. Surprisingly, abnormal bacteria are able to traffic to the endoplasmic reticulum, the usual replication niche of B. abortus in host cells. We also found that CtrA depletion affected outer membrane composition, in particular the abundance and spatial distribution of Omp25. Control of the B. abortus envelope composition by CtrA indicates the plasticity of the CtrA regulon along evolution.
langue originale | Anglais |
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Pages (de - à) | 780-797 |
Nombre de pages | 18 |
journal | Molecular Microbiology |
Volume | 103 |
Numéro de publication | 5 |
Date de mise en ligne précoce | 2016 |
Les DOIs | |
Etat de la publication | Publié - mars 2017 |
Empreinte digitale
Examiner les sujets de recherche de « CtrA Controls Cell Division and Outer Membrane Composition of the Pathogen Brucella abortus ». Ensemble, ils forment une empreinte digitale unique.-
PDR (FRSM) BRUCELL-CYCLE: BRUCELL-CYCLE
De Bolle, X. (Responsable du Projet), Willemart, K. (Technicien) & ONG, T. A. P. (Chercheur)
31/12/14 → …
Projet: Recherche
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PAI n°P7/28 - MICRODEV: Role of developmental processes in the virulence of human pathogens : from molecular mechanisms to novel therapeutic targets
LETESSON, J.-J. (Co-investigateur), Coenye, T. (Responsable du Projet), Van Dijck, P. (Co-investigateur), Van Melderen, L. (Co-investigateur), HOLS, P. (Co-investigateur) & De Bolle, X. (Co-investigateur)
1/10/12 → 30/09/17
Projet: Recherche
Équipement
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Laboratoire de sécurité biologique au niveau 3 (BL3)
De Bolle, X. (!!Manager)
Plateforme technologique Laboratoire de securite biologique au niveau 3Equipement/installations: Plateforme technolgique
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Morphologie - Imagerie
Cecchet, F. (!!Manager) & Renard, H.-F. (!!Manager)
Plateforme technologique Morphologie, imagerieEquipement/installations: Plateforme technolgique
Thèses de l'étudiant
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CtrA-dependant cell cycle regulation of Brucella abortus in culture and during infection
Francis, N. (Auteur), De Bolle, X. (Promoteur), Renard, P. (Président), Crosson, S. (Jury), Biondi, E. (Jury) & Collet, J.-F. (Jury), 1 déc. 2015Student thesis: Doc types › Docteur en Sciences
Fichier -
Study of cell cycle regulators and DNA repair following alkylating stress in Brucella abortus
Poncin, K. (Auteur), De Bolle, X. (Promoteur), Hallez, R. (Président), VIOLLIER, P. (Jury), Matic, I. (Jury) & Hallet, B. (Jury), 11 déc. 2018Student thesis: Doc types › Docteur en Sciences
Fichier