Résumé
Ways to monitor dabigatran etexilate (DE) therapy would be useful in certain situations. Functional assays such as aPTT or Hemoclot® Thrombin Inhibitor (HTI) have been proposed to evaluate dabigatran concentrations, but previous findings are based on in vitro studies and results must be confirmed in clinical samples. The aim of this study was to compare aPTT and HTI measurements with liquid chromatography- tandem mass spectrometry (LC-MS/MS) measurements of dabigatran in plasma samples from DE treated patients. Seventy-one plasma samples were included. aPTT was performed using STA-CKPrest® and SynthASil®. HTI was performed according to instructions from the manufacturer. The LC-MS/MS method utilised dabigatran- d3 as internal standard. The plasma concentration range was 0 to 645 ng/ml as measured by LC-MS/MS. Overall, the HTI and LC-MS/ MS analyses correlated well (r2=0.97). The Bland-Altman analysis showed a mean difference of 9 ng/ml (SD: 20 ng/ml). However, the HTI performed poorly at concentrations 50 ng/ml. Quantification of lower dabigatran levels in DE-treated patients requires the "reference" LC-MS/MS method.
langue originale | Anglais |
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Pages (de - à) | 543-549 |
Nombre de pages | 7 |
journal | Thrombosis and Haemostasis |
Volume | 110 |
Numéro de publication | 3 |
Les DOIs | |
Etat de la publication | Publié - sept. 2013 |
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Examiner les sujets de recherche de « Comparison of calibrated dilute thrombin time and aPTT tests with LC-MS/MS for the therapeutic monitoring of patients treated with dabigatran etexilate ». Ensemble, ils forment une empreinte digitale unique.Thèses de l'étudiant
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Evaluation of thrombotic and/or bleeding risks associated with anticoagulotherapy.
Douxfils, J. (Auteur)Dogne, J.-M. (Promoteur), Mullier, F. (Promoteur), Masereel, B. (Président), Chatelain, B. (Jury), Chatelain, C. (Jury), Verhamme, P. (Jury), TEN CATE, H. (Jury) & Ageno, W. (Jury), 24 oct. 2015Student thesis: Doc types › Docteur en Sciences Biomédicales et Pharmaceutiques