Compact Disc with both numeric and genomic information as DNA microarray platform

Isabelle Alexandre, Yves Houbion, Jérémy Collet, Sandrine Hamels, Joël Demarteau, Jean-Luc Gala, José Remacle

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

The Compact Disc (CD) is an ideal toll for reading, writing, and storing numéric information. It was used in this work as a support for constructing DNA microarrays suited for genomic analysis. The CD was divided into two functional areas : the external ring of the CD was used for multiparametric DNA analysis on arrays, and the inner portion was used for storing numeric information. Because polycarbonte and CD resins autofluoresce , a colorimetric method for DNA microarray detection was used that is welle adapted for the fast detection necassary when using a CD reader. A double-sided CD reader was developped for the simultaneous analysis of both array and numeric data. The numeric data are engraved as pits in the CD tracks and result in the sucession of 0/1, wich results from the modulation of the laser reflection when one reads the edges of the pits. Another diffraction-based laser was placed above the CD for the detection of the DNA targets on the microarrays. Both readers fit easily in a PC tower. Both numeric and genomic information data were simultaneously acquired, and each aray was reconstituted, analyzed, and processed for quantification by the appropriate software.
langue originaleAnglais
Pages (de - à)435-439
Nombre de pages5
journalBioTechniques
Volume33
Etat de la publicationPublié - 2002

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