Chloroquine allows to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes

R. Wattiaux, F. Gentinne, M. Jadot, F. Dubois, S. Wattiaux-De Coninck

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.

langue originaleAnglais
Pages (de - à)808-813
Nombre de pages6
journalBiochemical and Biophysical Research Communications
Volume190
Numéro de publication3
Les DOIs
étatPublié - 1 janv. 1993

Empreinte digitale

Chloroquine
Lysosomes
Hepatocytes
Cathepsin C
Liver
Rats
Injections
Arylsulfatases
Cellobiose
Hexosaminidases
Isopycnic Centrifugation
Tyramine
Centrifugation
Radioactivity
Bovine Serum Albumin
Acid Phosphatase
Endocytosis
Sucrose
Enzymes

Citer ceci

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Chloroquine allows to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes. / Wattiaux, R.; Gentinne, F.; Jadot, M.; Dubois, F.; Wattiaux-De Coninck, S.

Dans: Biochemical and Biophysical Research Communications, Vol 190, Numéro 3, 01.01.1993, p. 808-813.

Résultats de recherche: Contribution à un journal/une revueArticle

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T1 - Chloroquine allows to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes

AU - Wattiaux, R.

AU - Gentinne, F.

AU - Jadot, M.

AU - Dubois, F.

AU - Wattiaux-De Coninck, S.

PY - 1993/1/1

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N2 - We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.

AB - We have examined the effect of chloroquine on rat liver lysosomal enzyme distributions after isopycnic centrifugation in a sucrose gradient. Chloroquine injection causes the large majority of cathepsin C, acid phosphatase and N acetyl glucosaminidase to migrate towards lower density regions; on the other hand only about 50% of arylsulfatase and acid deoxyribonuclease are subjected to such a density shift. To specifically mark hepatocyte lysosomes and sinusoidal cell lysosomes, rats were injected with galactosylated bovine serum albumin (A) or mannosylated bovine serum albumin (B) labelled with 125I tyramine cellobiose; A is selectively endocytosed by hepatocytes, B by sinusoidal cells. The radioactivity distribution is affected by chloroquine in the same way as cathepsin C, after injection of A though it is not influenced by chloroquine after the injection of B. These results show that chloroquine does not modify the density of liver sinusoidal cell lysosomes when it decreases the density of hepatocyte lysosomes. Such a difference could result from the fact that sinusoidal cell lysosomes do not accumulate chloroquine to the same extent as hepatocyte lysosomes. Chloroquine treatment could be useful to distinguish between hepatocyte lysosomes and sinusoidal cell lysosomes.

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