Résumé
Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).
| langue originale | Anglais |
|---|---|
| Numéro d'article | 266 |
| journal | Journal of Nanoparticle Research |
| Volume | 19 |
| Numéro de publication | 8 |
| Les DOIs | |
| état | Publié - 1 août 2017 |
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Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use. / Laloy, Julie; Haguet, Hélène; Alpan, Lutfiye; Mancier, Valérie; Mejia, Jorge; Levi, Samuel; Dogné, Jean Michel; Lucas, Stéphane; Rousse, Céline; Fricoteaux, Patrick.
Dans: Journal of Nanoparticle Research, Vol 19, Numéro 8, 266, 01.08.2017.Résultats de recherche: Contribution à un journal/une revue › Article
TY - JOUR
T1 - Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use
AU - Laloy, Julie
AU - Haguet, Hélène
AU - Alpan, Lutfiye
AU - Mancier, Valérie
AU - Mejia, Jorge
AU - Levi, Samuel
AU - Dogné, Jean Michel
AU - Lucas, Stéphane
AU - Rousse, Céline
AU - Fricoteaux, Patrick
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).
AB - Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).
KW - Biomedicine
KW - Copper
KW - Core-shell nanopowders
KW - Procoagulant activity
KW - Silver
KW - Thrombin generation
UR - http://www.scopus.com/inward/record.url?scp=85026667447&partnerID=8YFLogxK
U2 - 10.1007/s11051-017-3937-0
DO - 10.1007/s11051-017-3937-0
M3 - Article
AN - SCOPUS:85026667447
VL - 19
JO - Journal of Nanoparticle Research
JF - Journal of Nanoparticle Research
SN - 1388-0764
IS - 8
M1 - 266
ER -