Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use

Julie Laloy, Hélène Haguet, Lutfiye Alpan, Valérie Mancier, Jorge Mejia, Samuel Levi, Jean Michel Dogné, Stéphane Lucas, Céline Rousse, Patrick Fricoteaux

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).

langueAnglais
Numéro d'article266
journalJournal of Nanoparticle Research
Volume19
Numéro8
Les DOIs
étatPublié - 1 août 2017

Empreinte digitale

Electrochemistry
Nanopowder
Platelets
Coagulation
Blood
Aggregation
Shell
Human
Agglomeration
hemolysis
coagulation
electrochemistry
platelets
blood
x rays
X-ray Spectroscopy
Nanoparticles
Metals
nanoparticles
metals

mots-clés

    Citer ceci

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    title = "Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use",
    abstract = "Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).",
    keywords = "Biomedicine, Copper, Core-shell nanopowders, Procoagulant activity, Silver, Thrombin generation",
    author = "Julie Laloy and H\{'e}l\{`e}ne Haguet and Lutfiye Alpan and Val\{'e}rie Mancier and Jorge Mejia and Samuel Levi and Dogn\{'e}, {Jean Michel} and St\{'e}phane Lucas and C\{'e}line Rousse and Patrick Fricoteaux",
    year = "2017",
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    doi = "10.1007/s11051-017-3937-0",
    language = "English",
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    journal = "Journal of Nanoparticle Research",
    issn = "1388-0764",
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    T1 - Characterization of core/shell Cu/Ag nanopowders synthesized by electrochemistry and assessment of their impact on hemolysis, platelet aggregation, and coagulation on human blood for potential wound dressing use

    AU - Laloy,Julie

    AU - Haguet,Hélène

    AU - Alpan,Lutfiye

    AU - Mancier,Valérie

    AU - Mejia,Jorge

    AU - Levi,Samuel

    AU - Dogné,Jean Michel

    AU - Lucas,Stéphane

    AU - Rousse,Céline

    AU - Fricoteaux,Patrick

    PY - 2017/8/1

    Y1 - 2017/8/1

    N2 - Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).

    AB - Copper/silver core/shell nanopowders with different metal ratio have been elaborated by electrochemistry (ultrasound-assisted electrolysis followed by a displacement reaction). Characterization was performed by several methods (X-ray diffraction, scanning electron microscope, energy-dispersive X-ray spectroscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, centrifugal liquid sedimentation, and zeta potential measurements). The mean diameter of all nanoparticles is around 10 nm. The impact of each nanopowder on hemolysis, platelet aggregation, and coagulation has been studied on whole human blood. Hemolysis assays were performed with spectrophotometric measurement and platelet aggregation, with light transmission aggregometry and was compared to Cu/Pt core/shell nanoparticles with similar size as negative control. Calibrated thrombin generation test has been used for a coagulation study. They neither impact platelet aggregation nor hemolysis and have a procoagulant effect whatever their composition (i.e., metal ratio). These results highlight that such nanopowders have a potential use in medical applications (e.g., wound dressing).

    KW - Biomedicine

    KW - Copper

    KW - Core-shell nanopowders

    KW - Procoagulant activity

    KW - Silver

    KW - Thrombin generation

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