TY - JOUR
T1 - Characterization of BoHV-5 field strains circulation and report of transient specific subtype of bovine herpesvirus 5 in Argentina
AU - Maidana, S.S.
AU - Ladelfa, M.F.
AU - Perez, S.E.
AU - Lomonaco, P.M.
AU - Del Medico Zajac, M.P.
AU - Odeon, A.
AU - Blanco Viera, F.J.
AU - Combessies, G.
AU - Fondevila, N.
AU - Palacios, M.
AU - Thiry, J.
AU - Muylkens, B.
AU - Thiry, E.
AU - Romera, S.A.
N1 - Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/2/7
Y1 - 2011/2/7
N2 - Background: Bovine herpesvirus 5 (BoHV-5) is a member of the subfamily Alphaherpesvirinae responsible for meningo-encephalitis in young cattle. The first case of bovine meningo-encephalitis associated with a herpesvirus infection was reported in Australia. The current geographical distribution of BoHV-5 infection is mainly restricted to South America, especially Brazil and Argentina. Outbreaks of BoHV-5 are regularly observed in Argentina suggesting the circulation of the virus in the bovine population. Results: Seventeen field strains of BoHV-5 isolated from 1984 to now were confirmed by differential PCR and subjected to restriction endonuclease analysis (REA). Viral DNA was cleaved with BstEII which allows differentiation among subtypes a, b and non a, non b. According to the REA with BstEII, only one field strain showed a pattern similar to the Argentinean A663 strain (prototype of BoHV-5b). All other isolates showed a clear pattern similar to the Australian N569 strain (prototype of BoHV-5a) consistent with the subtypes observed in Brazil, the other South-American country where BoHV-5 is known to be prevalent. The genomic region of subtype b responsible for the distinct pattern was determined and amplified by PCR; specifically a point mutation was identified on the restriction site of BstEII, which generates the profile specific of BoHV-5b. Conclusions: This is the first report of circulation of BoHV-5a in Argentina as the prevailing subtype. Therefore the circulation of BoHV-5b was restricted to a few years in Argentina, speculating that this subtype was not able to be maintained in the bovine population. The mutation in the gB gene is associated with a difference in the restriction patterns between subtypes "a" and "b".
AB - Background: Bovine herpesvirus 5 (BoHV-5) is a member of the subfamily Alphaherpesvirinae responsible for meningo-encephalitis in young cattle. The first case of bovine meningo-encephalitis associated with a herpesvirus infection was reported in Australia. The current geographical distribution of BoHV-5 infection is mainly restricted to South America, especially Brazil and Argentina. Outbreaks of BoHV-5 are regularly observed in Argentina suggesting the circulation of the virus in the bovine population. Results: Seventeen field strains of BoHV-5 isolated from 1984 to now were confirmed by differential PCR and subjected to restriction endonuclease analysis (REA). Viral DNA was cleaved with BstEII which allows differentiation among subtypes a, b and non a, non b. According to the REA with BstEII, only one field strain showed a pattern similar to the Argentinean A663 strain (prototype of BoHV-5b). All other isolates showed a clear pattern similar to the Australian N569 strain (prototype of BoHV-5a) consistent with the subtypes observed in Brazil, the other South-American country where BoHV-5 is known to be prevalent. The genomic region of subtype b responsible for the distinct pattern was determined and amplified by PCR; specifically a point mutation was identified on the restriction site of BstEII, which generates the profile specific of BoHV-5b. Conclusions: This is the first report of circulation of BoHV-5a in Argentina as the prevailing subtype. Therefore the circulation of BoHV-5b was restricted to a few years in Argentina, speculating that this subtype was not able to be maintained in the bovine population. The mutation in the gB gene is associated with a difference in the restriction patterns between subtypes "a" and "b".
UR - http://www.scopus.com/inward/record.url?scp=79551627050&partnerID=8YFLogxK
U2 - 10.1186/1746-6148-7-8
DO - 10.1186/1746-6148-7-8
M3 - Article
AN - SCOPUS:79551627050
SN - 1746-6148
SP - 8
JO - BMC Veterinary Research
JF - BMC Veterinary Research
ER -