Carbohydrate-Lectin Interaction on Graphene-Coated Surface Plasmon Resonance (SPR) Interfaces

Abra Penezic, Geetanjali Deokar, Dominique Vignaud, Emmanuelle Pichonat, Henri Happy, Palaniappan Subramanian, Blaženka Gasparović, Rabah Boukherroub, Sabine Szunerits

Résultats de recherche: Contribution à un journal/une revueArticleRevue par des pairs

Résumé

The paper describes the detection of carbohydrate-lectin interaction on graphene-on-metal surface plasmon resonance (SPR) interfaces. Graphene-coated gold-based SPR interfaces were formed through the transfer of large-area graphene grown by chemical vapor deposition (CVD) on polycrystalline Cu foils. The method allowed successful transfer of single- and double-layered graphene sheets onto the SPR interfaces in a reproducible manner. Functionalization of the graphene interface with mannose was achieved by simple immersion into a mannose aqueous solution (100 mM), resulting in noncovalent interactions between the aromatic ring structure of graphene and mannose. The utility of the carbohydrate-modified graphene-on-gold interface for the selective and sensitive detection of carbohydrate-lectin interactions was demonstrated using model lectins from Lens culinaris (LC) and Triticum vulgaris (TV). While LC lectin binds specifically to mannopyranoside units, TV lectin has an affinity for N-acetyl glucosamine and sialic acid residues.

langue originaleAnglais
Pages (de - à)677-683
Nombre de pages7
journalPlasmonics
Volume9
Numéro de publication3
Les DOIs
Etat de la publicationPublié - 2014
Modification externeOui

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