Brucella central carbon metabolism: an update

T Barbier, A Zúñiga-Ripa, S Moussa, H Plovier, J F Sternon, L Lázaro-Antón, Raquel Conde-Álvarez, X De Bolle, Maite Iriarte, Ignacio Moriyón, J J Letesson

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

The brucellae are facultative intracellular pathogens causing brucellosis, an important zoonosis. Here, we review the nutritional, genetic, proteomic and transcriptomic studies on Brucella carbon uptake and central metabolism, information that is needed for a better understanding of Brucella virulence. There is no uniform picture across species but the studies suggest primary and/or secondary transporters for unknown carbohydrates, lactate, glycerol phosphate, erythritol, xylose, ribose, glucose and glucose/galactose, and routes for their incorporation to central metabolism, including an erythritol pathway feeding the pentose phosphate cycle. Significantly, all brucellae lack phosphoenolpyruvate synthase and phosphofructokinase genes, which confirms previous evidence on glycolysis absence, but carry all Entner-Doudoroff (ED) pathway and Krebs cycle (and glyoxylate pathway) genes. However, glucose catabolism proceeds through the pentose phosphate cycle in the classical species, and the ED pathway operates in some rodent-associated brucellae, suggesting an ancestral character for this pathway in this group. Gluconeogenesis is functional but does not rely exclusively on classical fructose bisphosphatases. Evidence obtained using infection models is fragmentary but suggests the combined or sequential use of hexoses/pentoses, amino acids and gluconeogenic substrates. We also discuss the role of the phosphotransferase system, stringent reponse, quorum sensing, BvrR/S and sRNAs in metabolism control, an essential aspect of the life style of facultative intracellular parasites.

langueAnglais
Pages1-30
Nombre de pages30
journalCritical reviews in microbiology
Les DOIs
étatE-pub ahead of print - 2017
Modification externeOui

Empreinte digitale

Brucella
Carbon
Erythritol
Pentoses
Phosphates
Glucose
Genes
water dikinase pyruvate
Nutrigenomics
Phosphofructokinases
Quorum Sensing
Pentose Phosphate Pathway
Hexoses
Ribose
Citric Acid Cycle
Gluconeogenesis
Brucellosis
Zoonoses
Glycolysis
Fructose

mots-clés

    Citer ceci

    Barbier, T ; Zúñiga-Ripa, A ; Moussa, S ; Plovier, H ; Sternon, J F ; Lázaro-Antón, L ; Conde-Álvarez, Raquel ; De Bolle, X ; Iriarte, Maite ; Moriyón, Ignacio ; Letesson, J J. / Brucella central carbon metabolism: an update. Dans: Critical reviews in microbiology. 2017 ; p. 1-30
    @article{86457f55498245e996cca50ba6addda7,
    title = "Brucella central carbon metabolism: an update",
    abstract = "The brucellae are facultative intracellular pathogens causing brucellosis, an important zoonosis. Here, we review the nutritional, genetic, proteomic and transcriptomic studies on Brucella carbon uptake and central metabolism, information that is needed for a better understanding of Brucella virulence. There is no uniform picture across species but the studies suggest primary and/or secondary transporters for unknown carbohydrates, lactate, glycerol phosphate, erythritol, xylose, ribose, glucose and glucose/galactose, and routes for their incorporation to central metabolism, including an erythritol pathway feeding the pentose phosphate cycle. Significantly, all brucellae lack phosphoenolpyruvate synthase and phosphofructokinase genes, which confirms previous evidence on glycolysis absence, but carry all Entner-Doudoroff (ED) pathway and Krebs cycle (and glyoxylate pathway) genes. However, glucose catabolism proceeds through the pentose phosphate cycle in the classical species, and the ED pathway operates in some rodent-associated brucellae, suggesting an ancestral character for this pathway in this group. Gluconeogenesis is functional but does not rely exclusively on classical fructose bisphosphatases. Evidence obtained using infection models is fragmentary but suggests the combined or sequential use of hexoses/pentoses, amino acids and gluconeogenic substrates. We also discuss the role of the phosphotransferase system, stringent reponse, quorum sensing, BvrR/S and sRNAs in metabolism control, an essential aspect of the life style of facultative intracellular parasites.",
    keywords = "Journal Article",
    author = "T Barbier and A Z\{'u}\{~n}iga-Ripa and S Moussa and H Plovier and Sternon, {J F} and L L\{'a}zaro-Ant\{'o}n and Raquel Conde-\{'A}lvarez and {De Bolle}, X and Maite Iriarte and Ignacio Moriy\{'o}n and Letesson, {J J}",
    year = "2017",
    doi = "10.1080/1040841X.2017.1332002",
    language = "English",
    pages = "1--30",
    journal = "Critical reviews in microbiology",
    issn = "1040-841X",
    publisher = "Informa Healthcare",

    }

    Brucella central carbon metabolism: an update. / Barbier, T; Zúñiga-Ripa, A; Moussa, S; Plovier, H; Sternon, J F; Lázaro-Antón, L; Conde-Álvarez, Raquel; De Bolle, X; Iriarte, Maite; Moriyón, Ignacio; Letesson, J J.

    Dans: Critical reviews in microbiology, 2017, p. 1-30.

    Résultats de recherche: Contribution à un journal/une revueArticle

    TY - JOUR

    T1 - Brucella central carbon metabolism: an update

    AU - Barbier,T

    AU - Zúñiga-Ripa,A

    AU - Moussa,S

    AU - Plovier,H

    AU - Sternon,J F

    AU - Lázaro-Antón,L

    AU - Conde-Álvarez,Raquel

    AU - De Bolle,X

    AU - Iriarte,Maite

    AU - Moriyón,Ignacio

    AU - Letesson,J J

    PY - 2017

    Y1 - 2017

    N2 - The brucellae are facultative intracellular pathogens causing brucellosis, an important zoonosis. Here, we review the nutritional, genetic, proteomic and transcriptomic studies on Brucella carbon uptake and central metabolism, information that is needed for a better understanding of Brucella virulence. There is no uniform picture across species but the studies suggest primary and/or secondary transporters for unknown carbohydrates, lactate, glycerol phosphate, erythritol, xylose, ribose, glucose and glucose/galactose, and routes for their incorporation to central metabolism, including an erythritol pathway feeding the pentose phosphate cycle. Significantly, all brucellae lack phosphoenolpyruvate synthase and phosphofructokinase genes, which confirms previous evidence on glycolysis absence, but carry all Entner-Doudoroff (ED) pathway and Krebs cycle (and glyoxylate pathway) genes. However, glucose catabolism proceeds through the pentose phosphate cycle in the classical species, and the ED pathway operates in some rodent-associated brucellae, suggesting an ancestral character for this pathway in this group. Gluconeogenesis is functional but does not rely exclusively on classical fructose bisphosphatases. Evidence obtained using infection models is fragmentary but suggests the combined or sequential use of hexoses/pentoses, amino acids and gluconeogenic substrates. We also discuss the role of the phosphotransferase system, stringent reponse, quorum sensing, BvrR/S and sRNAs in metabolism control, an essential aspect of the life style of facultative intracellular parasites.

    AB - The brucellae are facultative intracellular pathogens causing brucellosis, an important zoonosis. Here, we review the nutritional, genetic, proteomic and transcriptomic studies on Brucella carbon uptake and central metabolism, information that is needed for a better understanding of Brucella virulence. There is no uniform picture across species but the studies suggest primary and/or secondary transporters for unknown carbohydrates, lactate, glycerol phosphate, erythritol, xylose, ribose, glucose and glucose/galactose, and routes for their incorporation to central metabolism, including an erythritol pathway feeding the pentose phosphate cycle. Significantly, all brucellae lack phosphoenolpyruvate synthase and phosphofructokinase genes, which confirms previous evidence on glycolysis absence, but carry all Entner-Doudoroff (ED) pathway and Krebs cycle (and glyoxylate pathway) genes. However, glucose catabolism proceeds through the pentose phosphate cycle in the classical species, and the ED pathway operates in some rodent-associated brucellae, suggesting an ancestral character for this pathway in this group. Gluconeogenesis is functional but does not rely exclusively on classical fructose bisphosphatases. Evidence obtained using infection models is fragmentary but suggests the combined or sequential use of hexoses/pentoses, amino acids and gluconeogenic substrates. We also discuss the role of the phosphotransferase system, stringent reponse, quorum sensing, BvrR/S and sRNAs in metabolism control, an essential aspect of the life style of facultative intracellular parasites.

    KW - Journal Article

    U2 - 10.1080/1040841X.2017.1332002

    DO - 10.1080/1040841X.2017.1332002

    M3 - Article

    SP - 1

    EP - 30

    JO - Critical reviews in microbiology

    T2 - Critical reviews in microbiology

    JF - Critical reviews in microbiology

    SN - 1040-841X

    ER -