Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

Background. Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA®-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component. Materials and methods. Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: The degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA®-Procoag-PPL assays) were assessed. Results. The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA®-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed. Discussion. The STA®-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

langueAnglais
Pages163-172
Nombre de pages10
journalBlood transfusion = Trasfusione del sangue
Volume16
Numéro2
Date de mise en ligne précoce2017
Les DOIs
étatPublié - févr. 2018
Modification externeOui

Empreinte digitale

Erythrocytes
Thrombin
Phospholipids
Erythrocyte Transfusion
Spectrophotometry
Hemolysis
Flow Cytometry
Extracellular Vesicles

mots-clés

    Citer ceci

    @article{11560ce46829432590fa4059e1c711c3,
    title = "Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates",
    abstract = "Background. Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA{\circledR}-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component. Materials and methods. Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: The degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA{\circledR}-Procoag-PPL assays) were assessed. Results. The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA{\circledR}-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed. Discussion. The STA{\circledR}-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.",
    keywords = "Coagulation, Extracellular vesicles, Red blood cell concentrates, Storage",
    author = "B{\'e}rang{\`e}re Devalet and Adeline Wannez and Nicolas Bailly and Lutfiye Alpan and Damien Gheldof and Jonathan Douxfils and V{\'e}ronique Deneys and Beno{\^i}t Bihin and Bernard Chatelain and Jean-Michel Dogn{\'e} and Christian Chatelain and Fran{\cc}ois Mullier",
    year = "2018",
    month = "2",
    doi = "10.2450/2017.0230-16",
    language = "English",
    volume = "16",
    pages = "163--172",
    journal = "Blood transfusion = Trasfusione del sangue",
    issn = "1723-2007",
    publisher = "SIMTI Servizi",
    number = "2",

    }

    TY - JOUR

    T1 - Application of a clot-based assay to measure the procoagulant activity of stored allogeneic red blood cell concentrates

    AU - Devalet, Bérangère

    AU - Wannez, Adeline

    AU - Bailly, Nicolas

    AU - Alpan, Lutfiye

    AU - Gheldof, Damien

    AU - Douxfils, Jonathan

    AU - Deneys, Véronique

    AU - Bihin, Benoît

    AU - Chatelain, Bernard

    AU - Dogné, Jean-Michel

    AU - Chatelain, Christian

    AU - Mullier, François

    PY - 2018/2

    Y1 - 2018/2

    N2 - Background. Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA®-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component. Materials and methods. Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: The degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA®-Procoag-PPL assays) were assessed. Results. The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA®-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed. Discussion. The STA®-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

    AB - Background. Thrombotic effects are possible complications of red blood cell transfusion. The generation and accumulation of procoagulant red blood cell extracellular vesicles during storage may play an important role in these thrombotic effects. The objective of this study was to assess the value of a simple phospholipid-dependent clot-based assay (STA®-Procoag-PPL) to estimate the procoagulant activity of stored red blood cells and changes in this activity during storage of the blood component. Materials and methods. Extracellular vesicles from 12 red blood cell concentrates were isolated at 13 storage time-points and characterised by quantitative and functional methods: The degree of haemolysis (direct spectrophotometry), the quantification and determination of cellular origin (flow cytometry) and the procoagulant activity (thrombin generation and STA®-Procoag-PPL assays) were assessed. Results. The mean clotting time of extracellular vesicles isolated from red blood cell concentrates decreased from 117.2±3.6 sec on the day of collection to 33.8±1.3 sec at the end of the storage period. This illustrates the phospholipid-dependent procoagulant activity of these extracellular vesicles, as confirmed by thrombin generation. Results of the peak of thrombin and the STA®-Procoag-PPL were well correlated (partial r=-0.41. p<0.001). In parallel, an exponential increase of the number of red blood cell-derived extracellular vesicles from 1,779/μL to 218,451/μL was observed. Discussion. The STA®-Procoag-PPL is a potentially useful technique for assessing the procoagulant activity of a red blood cell concentrate.

    KW - Coagulation

    KW - Extracellular vesicles

    KW - Red blood cell concentrates

    KW - Storage

    UR - http://www.scopus.com/inward/record.url?scp=85043531898&partnerID=8YFLogxK

    UR - http://www.mendeley.com/research/application-clotbased-assay-measure-procoagulant-activity-stored-allogeneic-red-blood-cell-concentra

    U2 - 10.2450/2017.0230-16

    DO - 10.2450/2017.0230-16

    M3 - Article

    VL - 16

    SP - 163

    EP - 172

    JO - Blood transfusion = Trasfusione del sangue

    T2 - Blood transfusion = Trasfusione del sangue

    JF - Blood transfusion = Trasfusione del sangue

    SN - 1723-2007

    IS - 2

    ER -