Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

R. Marega; L. Karmani; L. Flamant; P.G. Nageswaran; V. Valembois; B. Masereel; O. Feron; T. Vander Borght; S. Lucas; C. Michiels; B. Gallez; D. Bonifazi

doi:10.1039/c2jm33482h

Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

R. Marega, L. Karmani, L. Flamant, P.G. Nageswaran, V. Valembois, B. Masereel, O. Feron, T. Vander Borght, S. Lucas, C. Michiels, B. Gallez, D. Bonifazi

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Résumé

Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is

langue originale	Anglais
Pages (de - à)	21305-21312
Nombre de pages	8
journal	Journal of Materials Chemistry
Volume	22
Numéro de publication	39
Les DOIs	https://doi.org/10.1039/c2jm33482h
Etat de la publication	Publié - 2012

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THERAPLUS: Amélioration de la radiothérapie externe grâce à l'utilisation de nano-objets métalliques
Heuskin, A.-C. (Chercheur), Lucas, S. (Responsable du Projet), Michiels, C. (Co-investigateur), Feron, O. (Co-investigateur), Gallez, B. (Co-investigateur) & MARTINIVE, P. (Co-investigateur)
1/05/14 → 1/05/17
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TARGAN: Angiogénèse tumorale imagée et traitée à l'aide de nanoclusters radioactifs spécifiques.
Lucas, S. (Responsable du Projet), BOUCHAT, V. (Chercheur), CRESPIN, M. (Chercheur), Fransolet, M. (Technicien), Masereel, B. (Co-investigateur), Michiels, C. (Co-investigateur) & Moreau, N. (Chercheur)
1/03/06 → 31/08/11
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Microscopie électronique
Colomer, J.-F. (!!Manager) & Charlier, C. (!!Operator)
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Plateforme technologique Synthese, irradiation et analyse des materiaux
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15 mai 2014
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Contient cette citation

Marega, R., Karmani, L., Flamant, L., Nageswaran, P. G., Valembois, V., Masereel, B., Feron, O., Vander Borght, T., Lucas, S., Michiels, C., Gallez, B., & Bonifazi, D. (2012). Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study. Journal of Materials Chemistry, 22(39), 21305-21312. https://doi.org/10.1039/c2jm33482h

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title = "Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study",

abstract = "Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is",

author = "R. Marega and L. Karmani and L. Flamant and P.G. Nageswaran and V. Valembois and B. Masereel and O. Feron and {Vander Borght}, T. and S. Lucas and C. Michiels and B. Gallez and D. Bonifazi",

year = "2012",

doi = "10.1039/c2jm33482h",

language = "English",

volume = "22",

pages = "21305--21312",

journal = "Journal of Materials Chemistry",

publisher = "Royal Society of Chemistry",

number = "39",

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Marega, R, Karmani, L, Flamant, L, Nageswaran, PG, Valembois, V, Masereel, B , Feron, O, Vander Borght, T, Lucas, S , Michiels, C, Gallez, B & Bonifazi, D 2012, 'Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study', Journal of Materials Chemistry, VOL. 22, Numéro 39, p. 21305-21312. https://doi.org/10.1039/c2jm33482h

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T1 - Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

AU - Marega, R.

AU - Karmani, L.

AU - Flamant, L.

AU - Nageswaran, P.G.

AU - Valembois, V.

AU - Masereel, B.

AU - Feron, O.

AU - Vander Borght, T.

AU - Lucas, S.

AU - Michiels, C.

AU - Gallez, B.

AU - Bonifazi, D.

PY - 2012

Y1 - 2012

N2 - Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is

AB - Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is

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DO - 10.1039/c2jm33482h

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JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

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Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

Résumé

SDG des Nations Unies

Accès au document

Autres fichiers et liens

Empreinte digitale

Projets

THERAPLUS: Amélioration de la radiothérapie externe grâce à l'utilisation de nano-objets métalliques

TARGAN: Angiogénèse tumorale imagée et traitée à l'aide de nanoclusters radioactifs spécifiques.

Équipement

Microscopie électronique

Synthèse, Irradiation et Analyse de Matériaux (SIAM) (2016 - ...)

Activités

Theraplus project Kick-off Meeting

Contient cette citation