Résumé

Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is
langue originaleAnglais
Pages (de - à)21305-21312
Nombre de pages8
journalJournal of Materials Chemistry
Volume22
Numéro de publication39
Les DOIs
étatPublié - 2012

Empreinte digitale

Plasma deposition
Vapor deposition
Epidermal Growth Factor Receptor
Antibodies
Gold
Polymers
Nanoconjugates
Allylamine
Cells
Nanoparticles
Monoclonal antibodies
Radioisotopes
Assays
Monoclonal Antibodies
Doping (additives)
Plasmas
Epidermal Growth Factor

Citer ceci

@article{c27785ede7ad45fd981eb61a9711c62e,
title = "Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study",
abstract = "Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is",
author = "R. Marega and L. Karmani and L. Flamant and P.G. Nageswaran and V. Valembois and B. Masereel and O. Feron and {Vander Borght}, T. and S. Lucas and C. Michiels and B. Gallez and D. Bonifazi",
note = "Copyright 2012 Elsevier B.V., All rights reserved.",
year = "2012",
doi = "10.1039/c2jm33482h",
language = "English",
volume = "22",
pages = "21305--21312",
journal = "Journal of Materials Chemistry",
issn = "0959-9428",
publisher = "Royal Society of Chemistry",
number = "39",

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TY - JOUR

T1 - Antibody-functionalized polymer-coated gold nanoparticles targeting cancer cells: an in vitro and in vivo study

AU - Marega, R.

AU - Karmani, L.

AU - Flamant, L.

AU - Nageswaran, P.G.

AU - Valembois, V.

AU - Masereel, B.

AU - Feron, O.

AU - Vander Borght, T.

AU - Lucas, S.

AU - Michiels, C.

AU - Gallez, B.

AU - Bonifazi, D.

N1 - Copyright 2012 Elsevier B.V., All rights reserved.

PY - 2012

Y1 - 2012

N2 - Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is

AB - Gold nanoparticles (∼5 nm) coated with plasma-polymerized allylamine were produced through plasma vapor deposition and bioconjugated with a monoclonal antibody targeting the epidermal growth factor receptor. The resulting nanoconjugates displayed an antibody loading of about 1.7 nmol mg and efficiently target epidermal growth factor receptor overexpressing cell lines, as ascertained by ELISA and Western blot assays. The in vitro targeting properties were also confirmed in vivo, where a similar biodistribution profile of what was experienced for the unconjugated antibody was observed. Thanks to the possibility of doping the gold nanoparticles with radionuclides during plasma vapor deposition, the proposed functionalization strategy represents a very suitable platform for the in vivo cancer targeting with nanosized multifunctional particles. This journal is

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U2 - 10.1039/c2jm33482h

DO - 10.1039/c2jm33482h

M3 - Article

AN - SCOPUS:84870452285

VL - 22

SP - 21305

EP - 21312

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 39

ER -