Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li; Sandra Bouchy; Sebastien Penninckx; Riccardo Marega; Ornella Fichera; Bernard Gallez; Olivier Feron; Philippe Martinive; Anne-Catherine Heuskin; Carine Michiels; Stéphane Lucas

doi:10.2217/nnm-2018-0161

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li, Sandra Bouchy, Sebastien Penninckx, Riccardo Marega, Ornella Fichera, Bernard Gallez, Olivier Feron, Philippe Martinive, Anne-Catherine Heuskin, Carine Michiels, Stéphane Lucas

Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

Résumé

AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

langue originale	Anglais
Pages (de - à)	317-333
Nombre de pages	17
journal	Nanomedicine
Volume	14
Numéro de publication	3
Date de mise en ligne précoce	2019
Les DOIs	https://doi.org/10.2217/nnm-2018-0161
Etat de la publication	Publié - 1 févr. 2019

SDG des Nations Unies

Ce résultat contribue à ou aux Objectifs de développement durable suivants

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10.2217/nnm-2018-0161

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Link to publication in Scopus

Document sous embargo

2019_LiBouchyPenninckxMaregaFicheraHeuskinMichielsLucas_article
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40 Citations
1 Article

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties
Ternad, I., Penninckx, S., Lecomte, V., Vangijzegem, T., Conrard, L., Lucas, S., Heuskin, A-C., Michiels, C., Muller, R. N., Stanicki, D. & Laurent, S., 2 janv. 2023, Dans: Nanomaterials. 13, 1, 201.
Résultats de recherche: Contribution à un journal/une revue › Article › Revue par des pairs

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1 Terminé

THERAPLUS: Amélioration de la radiothérapie externe grâce à l'utilisation de nano-objets métalliques
Heuskin, A., Lucas, S., Michiels, C., Feron, O., Gallez, B. & MARTINIVE, P.
1/05/14 → 1/05/17
Projet: Recherche

Microscopie électronique
Jean-Francois Colomer (!!Manager) & Corry Charlier (!!Operator)
Plateforme technologique Morphologie, imagerie
Equipement/installations: Equipement
Morphologie - Imagerie
Francesca Cecchet (!!Manager) & Henri-Francois Renard (!!Manager)
Plateforme technologique Morphologie, imagerie
Equipement/installations: Plateforme technolgique
Synthèse, Irradiation et Analyse de Matériaux (SIAM) (2016 - ...)
Pierre Louette (!!Manager), Julien Colaux (!!Manager), Alexandre Felten (!!Manager), Tijani Tabarrant (!!Operator), Frederic COME (!!Operator) & Paul-Louis Debarsy (!!Manager)
Plateforme technologique Synthese, irradiation et analyse des materiaux
Equipement/installations: Plateforme technolgique

2 Participation à une conférence, un congrès

Advanced Radiotherapy Generated by Exploiting Nanoprocesses and Technologies international conference
Anne-Catherine Heuskin (Membre du Comité Scientifique)
22 janv. 2018 → 24 janv. 2018
Activité: Participation ou organisation d'un événement › Participation à une conférence, un congrès
Advanced Radiotherapy Generated by Exploiting Nanoprocesses and Technologies international conference
Anne-Catherine Heuskin (Orateur invité)
22 janv. 2018 → 24 janv. 2018
Activité: Participation ou organisation d'un événement › Participation à une conférence, un congrès

Contient cette citation

@article{42494735c1d945eabfbfcd0dbe631adb,

title = "Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy",

abstract = "AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.",

keywords = "EGFR, gold nanoparticles, nanoparticle uptake, protontherapy, radiosentizing effects, Microscopy, Electron, Transmission, Humans, Proton Therapy/methods, Metal Nanoparticles/chemistry, Cetuximab/chemistry, Gold/chemistry, Radiation-Sensitizing Agents/chemistry, Antibodies/chemistry, Cell Line, Tumor",

author = "Sha Li and Sandra Bouchy and Sebastien Penninckx and Riccardo Marega and Ornella Fichera and Bernard Gallez and Olivier Feron and Philippe Martinive and Anne-Catherine Heuskin and Carine Michiels and St{\'e}phane Lucas",

note = "Funding Information: The authors thank the {\textquoteleft}Morphology-Imaging{\textquoteright} technological platform of the University of Namur for the electron microscopy. The authors also acknowledge the support of the SIAM platform. S{\'e}bastien Penninckx is a PhD fellow funded by the Belgian Funds for Scientific Research (FRS-FNRS, Belgium). Funding Information: TheraPlus project was supported by the Walloon Region (grant 1318075). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: {\textcopyright} 2019 2019 Future Medicine Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",

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doi = "10.2217/nnm-2018-0161",

language = "English",

volume = "14",

pages = "317--333",

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T1 - Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

AU - Li, Sha

AU - Bouchy, Sandra

AU - Penninckx, Sebastien

AU - Marega, Riccardo

AU - Fichera, Ornella

AU - Gallez, Bernard

AU - Feron, Olivier

AU - Martinive, Philippe

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

AU - Lucas, Stéphane

N1 - Funding Information: The authors thank the ‘Morphology-Imaging’ technological platform of the University of Namur for the electron microscopy. The authors also acknowledge the support of the SIAM platform. Sébastien Penninckx is a PhD fellow funded by the Belgian Funds for Scientific Research (FRS-FNRS, Belgium). Funding Information: TheraPlus project was supported by the Walloon Region (grant 1318075). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Publisher Copyright: © 2019 2019 Future Medicine Ltd. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

AB - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

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KW - radiosentizing effects

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KW - Metal Nanoparticles/chemistry

KW - Cetuximab/chemistry

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KW - Radiation-Sensitizing Agents/chemistry

KW - Antibodies/chemistry

KW - Cell Line, Tumor

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SN - 1743-5889

VL - 14

SP - 317

EP - 333

JO - Nanomedicine

JF - Nanomedicine

IS - 3

ER -

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Résumé

SDG des Nations Unies

Accès au document

Autres fichiers et liens

Document sous embargo

Empreinte digitale

Résultat de recherche

Advances in the Mechanistic Understanding of Iron Oxide Nanoparticles' Radiosensitizing Properties

Projets

THERAPLUS: Amélioration de la radiothérapie externe grâce à l'utilisation de nano-objets métalliques

Équipement

Microscopie électronique

Morphologie - Imagerie

Synthèse, Irradiation et Analyse de Matériaux (SIAM) (2016 - ...)

Activités

Advanced Radiotherapy Generated by Exploiting Nanoprocesses and Technologies international conference

Advanced Radiotherapy Generated by Exploiting Nanoprocesses and Technologies international conference

Contient cette citation