Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Sha Li, Sandra Bouchy, Sebastien Penninckx, Riccardo Marega, Ornella Fichera, Bernard Gallez, Olivier Feron, Philippe Martinive, Anne-Catherine Heuskin, Carine Michiels, Stéphane Lucas

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Résumé

AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

langue originaleAnglais
Pages (de - à)317-333
Nombre de pages17
journalNanomedicine
Volume14
Numéro de publication3
Date de mise en ligne précoce2019
Les DOIs
Etat de la publicationPublié - 1 févr. 2019

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