Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

Résultats de recherche: Contribution à un journal/une revueArticle

Résumé

AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.

MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.

RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

langue originaleAnglais
Pages (de - à)317-333
Nombre de pages17
journalNanomedicine
Volume14
Numéro de publication3
Date de mise en ligne précoce2019
Les DOIs
étatPublié - 1 févr. 2019

Empreinte digitale

Proton Therapy
Antibodies
Gold
Nanoparticles
Protons
Tumors
Neoplasms
Proton irradiation
Cells
Radiation-Sensitizing Agents
Atomic spectroscopy
Transmission Electron Microscopy
Absorption spectroscopy
Assays
Spectrum Analysis
Transmission electron microscopy

Citer ceci

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title = "Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy",
abstract = "AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.",
keywords = "EGFR, gold nanoparticles, nanoparticle uptake, protontherapy, radiosentizing effects",
author = "Sha Li and Sandra Bouchy and Sebastien Penninckx and Riccardo Marega and Ornella Fichera and Bernard Gallez and Olivier Feron and Philippe Martinive and Anne-Catherine Heuskin and Carine Michiels and St{\'e}phane Lucas",
year = "2019",
month = "2",
day = "1",
doi = "10.2217/nnm-2018-0161",
language = "English",
volume = "14",
pages = "317--333",
journal = "Nanomedicine",
issn = "1743-5889",
publisher = "Future Medicine Ltd.",
number = "3",

}

Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy. / Li, Sha; Bouchy, Sandra; Penninckx, Sebastien; Marega, Riccardo; Fichera, Ornella; Gallez, Bernard; Feron, Olivier; Martinive, Philippe; Heuskin, Anne-Catherine; Michiels, Carine; Lucas, Stéphane.

Dans: Nanomedicine, Vol 14, Numéro 3, 01.02.2019, p. 317-333.

Résultats de recherche: Contribution à un journal/une revueArticle

TY - JOUR

T1 - Antibody-functionalized gold nanoparticles as tumor targeting radiosensitizers for proton therapy

AU - Li, Sha

AU - Bouchy, Sandra

AU - Penninckx, Sebastien

AU - Marega, Riccardo

AU - Fichera, Ornella

AU - Gallez, Bernard

AU - Feron, Olivier

AU - Martinive, Philippe

AU - Heuskin, Anne-Catherine

AU - Michiels, Carine

AU - Lucas, Stéphane

PY - 2019/2/1

Y1 - 2019/2/1

N2 - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

AB - AIM: This study aimed at developing antibody-functionalized gold nanoparticles (AuNPs) to selectively target cancer cells and probing their potential radiosensitizing effects under proton irradiation.MATERIALS & METHODS: AuNPs were conjugated with cetuximab (Ctxb-AuNPs). Ctxb-AuNP uptake was evaluated by transmission electron microscopy and atomic absorption spectroscopy. Radioenhancing effect was assessed using conventional clonogenic assay.RESULTS & CONCLUSION: Ctxb-AuNPs specifically bound to and accumulated in EGFR-overexpressing A431 cells, compared with EGFR-negative MDA-MB-453 cells. Ctxb-AuNPs enhanced the effect of proton irradiation in A431 cells but not in MDA-MB-453 cells. These data indicate, for the first time, that combining enhanced uptake by specific targeting and radioenhancing effect, using conjugated AuNPs, is a promising strategy to increase cell killing by protontherapy.

KW - EGFR

KW - gold nanoparticles

KW - nanoparticle uptake

KW - protontherapy

KW - radiosentizing effects

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