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Résumé
Abstract Background The evaluation of activated protein C (APC) resistance based on the endogenous thrombin potential (ETP) is recommended during the development of steroid contraceptives in women. In 2019, this assay was validated on the calibrated automated thrombogram (CAT) device. However, in view of its screening potential, its automation is essential. Objectives To transfer the ETP-based APC resistance assay on the ST Genesia system using reagent STG-ThromboScreen with exogenous APC added. Method Dose-response curves were performed to define APC concentration leading to 90 and interrun reproducibility was assessed. The normal range was defined on the basis of 56 samples from healthy individuals. The sensitivity was assessed on 40 samples from women using combined oral contraceptives (COCs). A method comparison with the validated ETP-based APC resistance on the CAT system was performed. Results were expressed in normalized APC sensitivity ratio (nAPCsr). Results The APC concentration leading to 9052 mU/mL. Intra- and interrun reproducibility showed standard deviation
langue originale | Anglais |
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Numéro d'article | e12684 |
Pages (de - à) | e12684 |
journal | Research and Practice in Thrombosis and Haemostasis |
Volume | 6 |
Numéro de publication | 3 |
Les DOIs | |
Etat de la publication | Publié - mars 2022 |
Empreinte digitale
Examiner les sujets de recherche de « Analytical performance of the endogenous thrombin potential–based activated protein C resistance assay on the automated ST Genesia system ». Ensemble, ils forment une empreinte digitale unique.Projets
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Clinical Pharmacology Research Group
Douxfils, J., Dogne, J., Musuamba Tshinanu, F., MASEREEL, B., Wieers, G., Haguet, H., RONVAUX, L., Donis, N., Morimont, L., Evrard, J., Siriez, R., Gillot, C., FAVRESSE, J., BOUVY, C., Djokoto, H., Didembourg, M., David, C., Melchionda, S., Maloteau, V., Boucher, A., Devel, P., Modaffari, E., Vandeputte, M., De Messemaeker, A., Decarpentrie, J., Vassart, J. & De Groote, A.
1/04/22 → …
Projet: Axe de recherche
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The HIC Project : Evaluation of hormone-induced coagulopathy
Dogne, J., Douxfils, J., Morimont, L., Didembourg, M. & Evrard, J.
1/09/19 → …
Projet: Recherche